Author Correction: Ionizing radiation modulates human macrophages towards a pro-inflammatory phenotype preserving their pro-invasive and pro-angiogenic capacities.



Teresa Pinto, Ana, Laranjeiro Pinto, Marta, Patrícia Cardoso, Ana, Monteiro, Cátia, Teixeira Pinto, Marta, Filipe Maia, André, Castro, Patrícia, Figueira, Rita, Monteiro, Armanda, Marques, Margarida
et al (show 6 more authors) (2022) Author Correction: Ionizing radiation modulates human macrophages towards a pro-inflammatory phenotype preserving their pro-invasive and pro-angiogenic capacities. Scientific reports, 12 (1). 4774-.

[thumbnail of Author Correction Ionizing radiation modulates human macrophages towards a pro-inflammatory phenotype preserving their pro-i.pdf] PDF
Author Correction Ionizing radiation modulates human macrophages towards a pro-inflammatory phenotype preserving their pro-i.pdf - Other

Download (685kB) | Preview

Abstract

This Article contains an error in the description of the data presented in Figure 2. Each blot demonstrating a protein of interest, or of its phosphorylated form, is matched with the expression of β-actin, used as loading control. The majority of the proteins were separated in different gels, apart from proteins p105, p50 and Bcl-xL which were run in the same gel and have the same loading control. As a result, the Figure 2 legend, “Ionizing radiation induces macrophage NF-κB activation and increases Bcl-xL expression. (A) Evaluation of RelA phosphorylation (Ser536) and RelB, cRel, p100/p52 and p105/p50 subunit expression, 1 and 6 h after irradiation (2, 6 and 10 Gy). (B) RelB nuclear translocation 6 h after macrophage irradiation (10 Gy). Histone deacetylase 1 (HDAC1) and β-actin were used as loading controls for nuclear and cytoplasmic fractions, respectively. (C) Evaluation of Bcl2 and Bcl-xL expression after macrophage irradiation. Western blot images are representative of protein expression/phosphorylation status in distinct donors (at least n = 4), evaluated in two independent experiments.” should read: “Ionizing radiation induces macrophage NF-κB activation and increases Bcl-xL expression. (A) Evaluation of RelA phosphorylation (Ser536) and RelB, cRel, p100/p52 and p105/p50 subunit expression, 1 and 6 h after irradiation (2, 6 and 10 Gy). (B) RelB nuclear translocation 6 h after macrophage irradiation (10 Gy). Histone deacetylase 1 (HDAC1) and β-actin were used as loading controls for nuclear and cytoplasmic fractions, respectively. (C) Evaluation of Bcl2 and Bcl-xL expression after macrophage irradiation. Western blot images are representative of protein expression/phosphorylation status in distinct donors (at least n = 4), evaluated in two independent experiments. The β-actin loading control of the panels comprised by p105, p50 (2A) and Bcl-xL (2C) is the same, since proteins were separated in the same gel electrophoresis.”

Item Type: Article
Uncontrolled Keywords: 3107 Microbiology, 31 Biological Sciences, 32 Biomedical and Clinical Sciences
Divisions: Faculty of Health and Life Sciences
Faculty of Health and Life Sciences > Institute of Systems, Molecular and Integrative Biology
Depositing User: Symplectic Admin
Date Deposited: 29 Sep 2023 09:38
Last Modified: 22 Jun 2024 17:45
DOI: 10.1038/s41598-022-08498-1
Related URLs:
URI: https://livrepository.liverpool.ac.uk/id/eprint/3173209