Understanding the Degradation of Core-Shell Nanogels Using Asymmetrical Flow Field Flow Fractionation

Niezabitowska, Edyta, Gray, Dominic MM, Gallardo-Toledo, Eduardo, Owen, Andrew ORCID: 0000-0002-9819-7651, Rannard, Steve PP ORCID: 0000-0002-6946-1097 and McDonald, Tom OO ORCID: 0000-0002-9273-9173
(2023) Understanding the Degradation of Core-Shell Nanogels Using Asymmetrical Flow Field Flow Fractionation. JOURNAL OF FUNCTIONAL BIOMATERIALS, 14 (7). 346-.

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Nanogels are candidates for biomedical applications, and core-shell nanogels offer the potential to tune thermoresponsive behaviour with the capacity for extensive degradation. These properties were achieved by the combination of a core of poly(N-isopropylmethacrylamide) and a shell of poly(N-isopropylacrylamide), both crosslinked with the degradable crosslinker N,N'-bis(acryloyl)cystamine. In this work, the degradation behaviour of these nanogels was characterised using asymmetric flow field flow fractionation coupled with multi-angle and dynamic light scattering. By monitoring the degradation products of the nanogels in real-time, it was possible to identify three distinct stages of degradation: nanogel swelling, nanogel fragmentation, and nanogel fragment degradation. The results indicate that the core-shell nanogels degrade slower than their non-core-shell counterparts, possibly due to a higher degree of self-crosslinking reactions occurring in the shell. The majority of the degradation products had molecule weights below 10 kDa, which suggests that they may be cleared through the kidneys. This study provides important insights into the design and characterisation of degradable nanogels for biomedical applications, highlighting the need for accurate characterisation techniques to measure the potential biological impact of nanogel degradation products.

Item Type: Article
Uncontrolled Keywords: asymmetrical flow field flow fractionation, core-shell, degradation, nanogel, poly(N-isopropylmethacrylamide), thermoresponsive
Divisions: Faculty of Health and Life Sciences
Faculty of Science and Engineering > School of Physical Sciences
Faculty of Health and Life Sciences > Institute of Systems, Molecular and Integrative Biology
Depositing User: Symplectic Admin
Date Deposited: 29 Sep 2023 15:59
Last Modified: 02 Oct 2023 09:17
DOI: 10.3390/jfb14070346
Open Access URL: https://doi.org/10.3390/jfb14070346
Related URLs:
URI: https://livrepository.liverpool.ac.uk/id/eprint/3173245