Anderson, James R 
ORCID: 0000-0003-0489-7997, Johnson, Emily ORCID: 0000-0002-3144-9914, Jenkins, Rosalind, Jacobsen, Stine, Green, Daniel, Walters, Marie, Bundgaard, Louise, Hausmans, Bas AC, van den Akker, Guus, Welting, Tim JM et al (show 6 more authors)
(2023)
Multi-Omic Temporal Landscape of Plasma and Synovial Fluid-Derived Extracellular Vesicles Using an Experimental Model of Equine Osteoarthritis
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 24 (19).
14888-.
ISSN 1661-6596, 1422-0067
Abstract
Extracellular vesicles (EVs) contribute to osteoarthritis pathogenesis through their release into joint tissues and synovial fluid. Synovial fluid-derived EVs have the potential to be direct biomarkers in the causal pathway of disease but also enable understanding of their role in disease progression. Utilizing a temporal model of osteoarthritis, we defined the changes in matched synovial fluid and plasma-derived EV small non-coding RNA and protein cargo using sequencing and mass spectrometry. Data exploration included time series clustering, factor analysis and gene enrichment interrogation. Chondrocyte signalling was analysed using luciferase-based transcription factor activity assays. EV protein cargo appears to be more important during osteoarthritis progression than small non-coding RNAs. Cluster analysis revealed plasma-EVs represented a time-dependent response to osteoarthritis induction associated with supramolecular complexes. Clusters for synovial fluid-derived EVs were associated with initial osteoarthritis response and represented immune/inflammatory pathways. Factor analysis for plasma-derived EVs correlated with day post-induction and were primarily composed of proteins modulating lipid metabolism. Synovial fluid-derived EVs factors represented intermediate filament and supramolecular complexes reflecting tissue repair. There was a significant interaction between time and osteoarthritis for CRE, NFkB, SRE, SRF with a trend for osteoarthritis synovial fluid-derived EVs at later time points to have a more pronounced effect.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | extracellular vesicles, osteoarthritis, multi-omics |
| Divisions: | Faculty of Health & Life Sciences Faculty of Health & Life Sciences > Inst. Life Courses & Medical Sciences Faculty of Health & Life Sciences > Inst. Infection, Vet & Ecological Sciences Faculty of Health & Life Sciences > Inst. Systems, Molec & Integrative Biology > Inst. Systems, Molec & Integrative Biology |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 17 Oct 2023 15:20 |
| Last Modified: | 16 Jun 2026 19:58 |
| DOI: | 10.3390/ijms241914888 |
| Open Access URL: | https://doi.org/10.3390/ijms241914888 |
| Related Websites: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3173835 |
| Disclaimer: | The University of Liverpool is not responsible for content contained on other websites from links within repository metadata. Please contact us if you notice anything that appears incorrect or inappropriate. |
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