Application of novel plasma separation filter cards for quantification of nucleoside/nucleotide reverse transcriptase inhibitor di/triphosphates in dried blood spot using LC-MS

Thompson, Beth, Dilly-Penchala, Sujan, Amara, Alieu ORCID: 0000-0002-1137-2948, Reynolds, Helen ORCID: 0000-0001-7443-4520, Khoo, Saye ORCID: 0000-0002-2769-0967 and Else, Laura (2023) Application of novel plasma separation filter cards for quantification of nucleoside/nucleotide reverse transcriptase inhibitor di/triphosphates in dried blood spot using LC-MS BIOANALYSIS, 15 (13). pp. 739-756. ISSN 1757-6180, 1757-6199

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Abstract

Background: A rapid and sensitive LC-MS method has been developed and validated for the quantification of nucleoside di/triphosphates using a novel plasma separation card (HemaSep). Materials & methods: Cards were spotted with whole blood and stored at -80°C. Metabolites were extracted using 70:30 MeOH:20% formic acid, followed by weak anion exchange SPE and eluted using a Biobasic-AX column. Quantification was performed using a triple quadrupole mass spectrometer with a calibration range of 1.25-250 pmol/sample. Results: The recovery of metabolites was high (>93%). Precision and accuracy were acceptable and metabolites remained stable on the card after 29 days (stored at ambient temperature). Conclusion: HemaSep dried blood spots are a useful microsampling tool and offer an alternative to liquid plasma as they maintain stability over time.

Item Type:	Article
Uncontrolled Keywords:	adherence, antiretrovirals, dried blood spots, emtricitabine triphosphate, lamivudine triphosphate, LC-MS, tenofovir diphosphate
Divisions:	Faculty of Health & Life Sciences Faculty of Health & Life Sciences > Inst. Systems, Molec & Integrative Biology > Inst. Systems, Molec & Integrative Biology
Depositing User:	Symplectic Admin
Date Deposited:	14 Dec 2023 08:59
Last Modified:	22 May 2026 16:20
DOI:	10.4155/bio-2023-0057
Open Access URL:	https://doi.org/10.4155/bio-2023-0057
Related Websites:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3177368
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