CBL/CAP Is Essential for Mitochondria Respiration Complex I Assembly and Bioenergetics Efficiency in Muscle Cells.


Aye, Cho-Cho, Hammond, Dean E, Rodriguez-Cuenca, Sergio, Doherty, Mary K, Whitfield, Phillip D, Phelan, Marie M, Yang, Chenjing, Perez-Perez, Rafael, Li, Xiaoxin, Diaz-Ramos, Angels et al (show 6 more authors) (2023) CBL/CAP Is Essential for Mitochondria Respiration Complex I Assembly and Bioenergetics Efficiency in Muscle Cells. International journal of molecular sciences, 24 (4). p. 3399. ISSN 1422-0067, 1422-0067

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Abstract

CBL is rapidly phosphorylated upon insulin receptor activation. Mice whole body CBL depletion improved insulin sensitivity and glucose clearance; however, the precise mechanisms remain unknown. We depleted either CBL or its associated protein SORBS1/CAP independently in myocytes and assessed mitochondrial function and metabolism compared to control cells. CBL- and CAP-depleted cells showed increased mitochondrial mass with greater proton leak. Mitochondrial respiratory complex I activity and assembly into respirasomes were reduced. Proteome profiling revealed alterations in proteins involved in glycolysis and fatty acid degradation. Our findings demonstrate CBL/CAP pathway couples insulin signaling to efficient mitochondrial respiratory function and metabolism in muscle.

Item Type: Article
Uncontrolled Keywords: CBL, SORBS1, mitochondria, insulin signalling, insulin resistance, glucose transport
Divisions: Faculty of Health & Life Sciences
Faculty of Health & Life Sciences > Inst. Systems, Molec & Integrative Biology > Inst. Systems, Molec & Integrative Biology
Depositing User: Symplectic Admin
Date Deposited: 14 Dec 2023 09:18
Last Modified: 23 May 2026 11:16
DOI: 10.3390/ijms24043399
Open Access URL: https://doi.org/10.3390/ijms24043399
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URI: https://livrepository.liverpool.ac.uk/id/eprint/3177381
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