Transcriptomic profiling of cerebrospinal fluid identifies ALS pathway enrichment and RNA biomarkers in MND individuals.

Fröhlich, Alexander, Pfaff, Abigail L, Bubb, Vivien J ORCID: 0000-0003-2763-7004, Quinn, John P ORCID: 0000-0003-3551-7803 and Koks, Sulev ORCID: 0000-0001-6087-6643 (2023) Transcriptomic profiling of cerebrospinal fluid identifies ALS pathway enrichment and RNA biomarkers in MND individuals. Experimental biology and medicine (Maywood, N.J.), 248 (23). p. 15353702231209427. ISSN 1535-3702, 1535-3699

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Abstract

Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disorder and the most common form of motor neurone disease (MND) which is characterized by the damage and death of motor neurons in the brain and spinal cord of affected individuals. Due to the heterogeneity of the disease, a better understanding of the interaction between genetics and biochemistry with the identification of biomarkers is crucial for therapy development. In this study, we used cerebrospinal fluid (CSF) RNA-sequencing data from the New York Genome Center (NYGC) ALS Consortium and analyzed differential gene expression between 47 MND individuals and 29 healthy controls. Pathway analysis showed that the affected genes are enriched in many pathways associated with ALS, including nucleocytoplasmic transport, autophagy, and apoptosis. Moreover, we assessed differential expression on both gene- and transcript-based levels and demonstrate that the expression of previously identified potential biomarkers, including CAPG, CCL3, and MAP2, was significantly higher in MND individuals. Ultimately, this study highlights the transcriptomic composition of CSF which enables insights into changes in the brain in ALS and therefore increases the confidence in the use of CSF for biomarker development.

Item Type:	Article
Uncontrolled Keywords:	Cerebrospinal fluid, amyotrophic lateral sclerosis, motor neurone disease, transcriptome, RNA-seq, biomarker
Divisions:	Faculty of Health & Life Sciences Faculty of Health & Life Sciences > Inst. Systems, Molec & Integrative Biology > Inst. Systems, Molec & Integrative Biology
Depositing User:	Symplectic Admin
Date Deposited:	09 Jan 2024 10:41
Last Modified:	28 Feb 2026 15:50
DOI:	10.1177/15353702231209427
Related Websites:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3177768
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