A subpopulation of Talin 1 resides in the nucleus and regulates gene expression

Da Silva, Alejandro, Hästbacka, Hendrik, Puustinen, Mikael, Pessa, Jenny ORCID: 0000-0002-9113-6784, Goult, Benjamin ORCID: 0000-0002-3438-2807, Jacquemet, Guillaume ORCID: 0000-0002-9286-920X, Henriksson, Eva ORCID: 0000-0001-8320-4591 and Sistonen, Lea
(2022) A subpopulation of Talin 1 resides in the nucleus and regulates gene expression. [Preprint]

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Talin 1 (TLN1) is best known for its role at focal adhesions, where it activates β-integrin receptors and transmits mechanical stimuli to the actin cytoskeleton. Interestingly, the localization of TLN1 is not restricted to the focal adhesions, but its function in other cellular compartments remains poorly understood. By utilizing both biochemical and confocal microscopy analyses, we show that TLN1 localizes to the nucleus and that it strongly interacts with the chromatin. Importantly, depletion of endogenous TLN1 results in extensive changes in the gene expression profile of human breast epithelial cells. To determine the impact of nuclear TLN1 on gene regulation, we expressed a TLN1 fusion protein containing a nuclear localization signal. Our results revealed that nuclear TLN1 regulates a specific subset of the TLN1-dependent genes. Taken together, we show that apart from localizing at the plasma membrane and cytoplasm, TLN1 also resides in the nucleus where it functions in the regulation of gene expression.

Item Type: Preprint
Uncontrolled Keywords: 3101 Biochemistry and Cell Biology, 31 Biological Sciences, Genetics, Biotechnology, Cancer, Women's Health, 1.1 Normal biological development and functioning
Divisions: Faculty of Health and Life Sciences
Faculty of Health and Life Sciences > Institute of Systems, Molecular and Integrative Biology
Depositing User: Symplectic Admin
Date Deposited: 13 Mar 2024 10:01
Last Modified: 19 Jul 2024 16:56
DOI: 10.1101/2022.03.15.484419
Open Access URL: https://doi.org/10.1101/2022.03.15.484419
Related URLs:
URI: https://livrepository.liverpool.ac.uk/id/eprint/3179304