Single-channel digital LAMP multiplexing using amplification curve analysis.


Malpartida-Cardenas, Kenny, Miglietta, Luca, Peng, Tianyi, Moniri, Ahmad, Holmes, Alison ORCID: 0000-0001-5554-5743, Georgiou, Pantelis and Rodriguez-Manzano, Jesus (2022) Single-channel digital LAMP multiplexing using amplification curve analysis. Sensors & diagnostics, 1 (3). pp. 465-468. ISSN 2635-0998, 2635-0998

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Abstract

Loop-mediated isothermal amplification assays are currently limited to one target per reaction in the absence of melting curve analysis, molecular probes or restriction enzyme digestion. Here, we demonstrate multiplexing of five targets in a single fluorescent channel using digital LAMP and the machine learning-based method amplification curve analysis, resulting in a classification accuracy of 91.33% on 54 186 positive amplification events.

Item Type: Article
Uncontrolled Keywords: 46 Information and Computing Sciences, 3101 Biochemistry and Cell Biology, 31 Biological Sciences, Machine Learning and Artificial Intelligence, Networking and Information Technology R&D (NITRD)
Divisions: Faculty of Health and Life Sciences
Faculty of Health and Life Sciences > Institute of Systems, Molecular and Integrative Biology
Depositing User: Symplectic Admin
Date Deposited: 09 Apr 2024 09:58
Last Modified: 15 Aug 2025 09:34
DOI: 10.1039/d2sd00038e
Open Access URL: https://doi.org/10.1039/D2SD00038E
Related Websites:
URI: https://livrepository.liverpool.ac.uk/id/eprint/3180210
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