A novel LC-MS/MS method for the determination of favipiravir ribofuranosyl-5'-triphosphate (T-705-RTP) in human peripheral mononuclear cells.

Challenger, Elizabeth ORCID: 0000-0002-8978-6067, Dilly-Penchala, Sujan, Hale, Colin, Fitzgerald, Richard, Reynolds, Helen ORCID: 0000-0001-7443-4520, Chiong, Justin ORCID: 0000-0001-8063-6399, Rowland, Tim, Fletcher, Tom, Khoo, Saye ORCID: 0000-0002-2769-0967 and Else, Laura (2024) A novel LC-MS/MS method for the determination of favipiravir ribofuranosyl-5'-triphosphate (T-705-RTP) in human peripheral mononuclear cells. Journal of pharmaceutical and biomedical analysis, 245. p. 116155. ISSN 0731-7085, 1873-264X

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Abstract

Favipiravir is a broad-spectrum antiviral that is metabolised intracellularly into the active form, favipiravir ribofuranosyl-5'-triphosphate (F-RTP). Measurement of the intracellular concentration of F-RTP in mononuclear cells is a crucial step to characterising the pharmacokinetics of F-RTP and to enable more appropriate dose selection for the treatment of COVID-19 and emerging infectious diseases. The described method was validated over the range 24 - 2280 pmol/sample. Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood and lysed using methanol-water (70:30, v/v) before cellular components were precipitated with acetonitrile and the supernatant further cleaned by weak anion exchange solid phase extraction. The method was found to be both precise and accurate and was successfully utilised to analyse F-RTP concentrations in patient samples collected as part of the AGILE CST-6 clinical trial.

Item Type:	Article
Uncontrolled Keywords:	T-705 RTP, Favipiravir RTP, SARS-CoV-2, COVID-19, Bioanalysis
Divisions:	Faculty of Health & Life Sciences Faculty of Health & Life Sciences > Inst. Systems, Molec & Integrative Biology > Inst. Systems, Molec & Integrative Biology
Depositing User:	Symplectic Admin
Date Deposited:	29 Apr 2024 08:03
Last Modified:	22 May 2026 21:12
DOI:	10.1016/j.jpba.2024.116155
Open Access URL:	https://doi.org/10.1016/j.jpba.2024.116155
Related Websites:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3180619
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