Membrane Proteins in Action Monitored by pH-Responsive Liquid Crystal Biosensors.

Bao, Peng ORCID: 0000-0002-7875-7420, Phillips, Kyle and Raval, Rasmita (2024) Membrane Proteins in Action Monitored by pH-Responsive Liquid Crystal Biosensors. ACS applied materials & interfaces, 16 (24). pp. 31843-31850. ISSN 1944-8244, 1944-8252

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Abstract

Liquid crystal (LC) biosensors have received significant attention for their potential applications for point-of-care devices due to their sensitivity, low cost, and easy read-out. They have been employed to detect a wide range of important biological molecules. However, detecting the function of membrane proteins has been extremely challenging due to the difficulty of integrating membrane proteins, lipid membranes, and LCs into one system. In this study, we addressed this challenge by monitoring the proton-pumping function of bacteriorhodopsin (bR) using a pH-sensitive LC thin film biosensor. To achieve this, we deposited purple membranes (PMs) containing a 2D crystal form of bRs onto an LC-aqueous interface. Under light, the PM patches changed the local pH at the LC-aqueous interface, causing a color change in the LC thin film that is observable through a polarizing microscope with crossed polarizers. These findings open up new opportunities to study the biofunctions of membrane proteins and their induced local environmental changes in a solution using LC biosensors.

Item Type:	Article
Uncontrolled Keywords:	Purple Membrane, Bacteriorhodopsins, Membrane Proteins, Biosensing Techniques, Hydrogen-Ion Concentration, Liquid Crystals
Depositing User:	Symplectic Admin
Date Deposited:	19 Jun 2024 15:21
Last Modified:	08 Dec 2024 03:07
DOI:	10.1021/acsami.4c06614
Open Access URL:	https://pubs.acs.org/doi/epdf/10.1021/acsami.4c066...
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3182306