Maestri, Alice, Pons, Benoit J, Pursey, Elizabeth, Chong, Charlotte E, Gandon, Sylvain, Custodio, Rafael, Olina, Anna, Agapov, Aleksei, Chisnall, Matthew AW, Grasso, Anita et al (show 6 more authors)
(2024)
The bacterial defense system MADS interacts with CRISPR-Cas to limit phage infection and escape.
Cell Host & Microbe, 32 (8).
1412-1426.e11.
Abstract
The constant arms race between bacteria and their parasites has resulted in a large diversity of bacterial defenses, with many bacteria carrying multiple systems. Here, we report the discovery of a phylogenetically widespread defense system, coined methylation-associated defense system (MADS), which is distributed across gram-positive and gram-negative bacteria. MADS interacts with a CRISPR-Cas system in its native host to provide robust and durable resistance against phages. While phages can acquire epigenetic-mediated resistance against MADS, co-existence of MADS and a CRISPR-Cas system limits escape emergence. MADS comprises eight genes with predicted nuclease, ATPase, kinase, and methyltransferase domains, most of which are essential for either self/non-self discrimination, DNA restriction, or both. The complex genetic architecture of MADS and MADS-like systems, relative to other prokaryotic defenses, points toward highly elaborate mechanisms of sensing infections, defense activation, and/or interference.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Bacteria, Gram-Negative Bacteria, Gram-Positive Bacteria, Bacteriophages, Bacterial Proteins, Phylogeny, Methylation, CRISPR-Cas Systems |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 02 Aug 2024 07:43 |
| Last Modified: | 24 Aug 2024 02:18 |
| DOI: | 10.1016/j.chom.2024.07.005 |
| Open Access URL: | https://www.sciencedirect.com/science/article/pii/... |
| Related URLs: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3183291 |
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