Label free tracking to quantify nanoparticle diffusion through biological media.

Schleyer, Genevieve ORCID: 0009-0005-4245-9703, Patterson, Eann A ORCID: 0000-0003-4397-2160 and Curran, Judith M (2024) Label free tracking to quantify nanoparticle diffusion through biological media. Scientific reports, 14 (1). p. 18822. ISSN 2045-2322, 2045-2322

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Abstract

Nanotechnology is a rapidly evolving field and has been extensively studied in biological applications. An understanding of the factors that influence nanoparticle diffusion in biofluids can aid in the development of diverse technologies. The development of real-time, label-free tracking technologies would allow the expansion of current knowledge of the diffusion and activity of nanoparticles. Fluorescence-based microscopy is one of the most widespread tools to monitor and track nanoparticle dynamics; however, the influence of fluorescent tags on diffusion and biological activity is still unclear. In this study, we experimentally determined the diffusion coefficient of gold nanoparticles using a label-free, optical tracking technique and evaluated the influence of protein concentration, charge and diameter on nanoparticle diffusion through biological media. We dispersed positively- and negatively-charged nanoparticles with diameters varying from 10 to 100 nm in a common cell culture media with different concentrations of serum proteins. Our results show that dynamic protein interactions influence nanoparticle diffusion in the range of serum concentrations tested. Experimental regimes to obtain quantitative information on the factors that influence the dynamics of nanoparticles in biological media have been developed.

Item Type:	Article
Uncontrolled Keywords:	Gold, Culture Media, Microscopy, Fluorescence, Diffusion, Particle Size, Nanoparticles, Metal Nanoparticles
Depositing User:	Symplectic Admin
Date Deposited:	19 Aug 2024 07:38
Last Modified:	08 Dec 2024 02:23
DOI:	10.1038/s41598-024-69506-0
Open Access URL:	https://www.nature.com/articles/s41598-024-69506-0...
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3183855