Ersoy, Ufuk, Altinpinar, Atilla Emre, Kanakis, Ioannis 
ORCID: 0000-0001-6410-1482, Alameddine, Moussira, Gioran, Anna, Chondrogianni, Niki, Ozanne, Susan E, Peffers, Mandy Jayne ORCID: 0000-0001-6979-0440, Jackson, Malcolm J ORCID: 0000-0003-3683-8297, Goljanek-Whysall, Katarzyna ORCID: 0000-0001-8166-8800 et al (show 1 more authors)
(2024)
Lifelong dietary protein restriction induces denervation and skeletal muscle atrophy in mice.
Free radical biology & medicine, 224.
S0891-5849(24)00648-8-S0891-5849(24)00648-8.
ISSN 0891-5849, 1873-4596
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1-s2.0-S0891584924006488-main.pdf - Open Access published version Download (12MB) | Preview |
Abstract
As a widespread global issue, protein deficiency hinders development and optimal growth in offspring. Maternal low-protein diet influences the development of age-related diseases, including sarcopenia, by altering the epigenome and organ structure through potential increase in oxidative stress. However, the long-term effects of lactational protein restriction or postnatal lifelong protein restriction on the neuromuscular system have yet to be elucidated. Our results demonstrated that feeding a normal protein diet after lactational protein restriction did not have significant impacts on the neuromuscular system in later life. In contrast, a lifelong low-protein diet induced a denervation phenotype and led to demyelination in the sciatic nerve, along with an increase in the number of centralised nuclei and in the gene expression of atrogenes at 18 months of age, indicating an induced skeletal muscle atrophy. These changes were accompanied by an increase in proteasome activity in skeletal muscle, with no significant alterations in oxidative stress or mitochondrial dynamics markers in skeletal muscle later in life. Thus, lifelong protein restriction may induce skeletal muscle atrophy through changes in peripheral nerves and neuromuscular junctions, potentially contributing to the early onset or exaggeration of sarcopenia.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Muscle, Skeletal, Neuromuscular Junction, Sciatic Nerve, Animals, Mice, Inbred C57BL, Mice, Muscular Atrophy, Proteasome Endopeptidase Complex, Diet, Protein-Restricted, Denervation, Oxidative Stress, Female, Male, Sarcopenia |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 16 Sep 2024 08:53 |
| Last Modified: | 18 Jan 2025 02:00 |
| DOI: | 10.1016/j.freeradbiomed.2024.09.005 |
| Related Websites: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3184516 |
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