Edge, Rebecca J 
ORCID: 0000-0001-8574-3724, Marriott, Amy E ORCID: 0000-0002-4806-9539, Stars, Emma L, Patel, Rohit N ORCID: 0000-0003-4070-3256, Wilkinson, Mark C, King, Lloyd DW, Slagboom, Julien ORCID: 0000-0003-2929-1086, Tan, Choo Hock ORCID: 0000-0003-1416-077X, Ratanabanangkoon, Kavi, Draper, Simon J ORCID: 0000-0002-9415-1357 et al (show 1 more authors)
(2024)
Plug and play virus-like particles for the generation of anti-toxin antibodies.
Toxicon: X, 23.
100204-.
ISSN 2590-1710, 2590-1710
Abstract
Snakebite is a major global health concern, for which antivenom remains the only approved treatment to neutralise the harmful effects of the toxins. However, some medically important toxins are poorly immunogenic, resulting in reduced efficacy of the final product. Boosting the immunogenicity of these toxins in the commercial antivenom immunising mixtures could be an effective strategy to improve the final dose efficacy, and displaying snake antigens on Virus-like particles (VLPs) is one method for this. However, despite some applications in the field of snakebite, VLPs have yet to be explored in methods that could be practical at an antivenom manufacturing scale. Here we describe the utilisation of a "plug and play" VLP system to display immunogenic linear peptide epitopes from three finger toxins (3FTxs) and generate anti-toxin antibodies. Rabbits were immunised with VLPs displaying individual consensus linear epitopes and their antibody responses were characterised by immunoassay. Of the three experimental consensus sequences, two produced antibodies capable of recognising the consensus peptides, whilst only one of these could also recognise native whole toxins. Further characterisation of antibodies raised against this peptide demonstrated a sub-class specific response, and that these were able to elicit partially neutralising antibody responses, resulting in increased survival times in a murine snakebite envenoming model.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | 32 Biomedical and Clinical Sciences, 3204 Immunology, Immunization, Vaccine Related, Biotechnology, 5.1 Pharmaceuticals, 3 Good Health and Well Being |
| Divisions: | Faculty of Health & Life Sciences Faculty of Health & Life Sciences > Inst. Infection, Vet & Ecological Sciences Faculty of Health & Life Sciences > Inst. Systems, Molec & Integrative Biology > Inst. Systems, Molec & Integrative Biology |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 18 Oct 2024 14:58 |
| Last Modified: | 20 Jan 2026 19:32 |
| DOI: | 10.1016/j.toxcx.2024.100204 |
| Open Access URL: | https://doi.org/10.1016/j.toxcx.2024.100204 |
| Related Websites: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3185210 |
| Disclaimer: | The University of Liverpool is not responsible for content contained on other websites from links within repository metadata. Please contact us if you notice anything that appears incorrect or inappropriate. |
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