Gelatin emulsion gels loaded with host defence peptides for the treatment of antibiotic-resistant infections.

Mann, Kiran, Aveyard, Jenny, Dallos Ortega, Mateo, Chen, Ting, Koduri, Manohar Prasad, Fothergill, Joanne L ORCID: 0000-0002-7012-1508, Schache, Andrew G ORCID: 0000-0001-9466-6038, Curran, Judith M, Poole, Robert J ORCID: 0000-0001-6686-4301 and D'Sa, Raechelle A ORCID: 0000-0003-2651-8783 (2025) Gelatin emulsion gels loaded with host defence peptides for the treatment of antibiotic-resistant infections. Biomaterials advances, 166. 214071-. ISSN 2772-9516, 2772-9508

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Official URL: https://doi.org/10.1016/j.bioadv.2024.214071

Abstract

The surge in multidrug-resistant bacteria against conventional antibiotics is a rapidly developing global health crisis necessitating novel infection management strategies. Host defence peptides (HDPs), also known as antimicrobial peptides (AMPs), offer a promising alternative to traditional antibiotics, but their practical translation is limited by their susceptibility to proteases and potential off-site cytotoxicity. In this paper, we investigate the feasibility of using gelatin emulsion gels (GELs), prepared using a water-in-oil (W/O) method, for the delivery of HDPs DJK-5 and IDR-1018 to improve their clinical utility. DJK-5-loaded GELs exhibited complete eradication of planktonic Methicillin-resistant Staphylococcus aureus (MRSA) at 4 - and 24-h intervals. Similarly, IDR-1018-loaded GELs demonstrated almost complete killing of MRSA and Escherichia coli (E. coli) after 4 h. Importantly, none of the GEL formulations investigated exhibited in vitro cytotoxicity. Overall, these HDP loaded GELs are a promising solution for the treatment of antibiotic-resistant infections.

Item Type:	Article
Uncontrolled Keywords:	Humans, Escherichia coli, Staphylococcal Infections, Antimicrobial Cationic Peptides, Gelatin, Emulsions, Gels, Anti-Bacterial Agents, Microbial Sensitivity Tests, Methicillin-Resistant Staphylococcus aureus, Antimicrobial Peptides
Divisions:	Faculty of Health and Life Sciences Faculty of Science and Engineering Faculty of Science and Engineering > School of Engineering Faculty of Health and Life Sciences > Institute of Infection, Veterinary and Ecological Sciences
Depositing User:	Symplectic Admin
Date Deposited:	01 Nov 2024 16:10
Last Modified:	08 Dec 2024 03:12
DOI:	10.1016/j.bioadv.2024.214071
Open Access URL:	https://doi.org/10.1016/j.bioadv.2024.214071
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3187010