Tsakiroglou, Maria 
ORCID: 0000-0001-7946-3953, Evans, Anthony ORCID: 0000-0001-8547-1730, Doce-Carracedo, Alejandra, Little, Margaret, Hornby, Rachel, Roberts, Paul, Zhang, Eunice ORCID: 0000-0003-1813-2207, Miyajima, Fabio and Pirmohamed, Munir ORCID: 0000-0002-7534-7266
(2024)
Gene Expression Dysregulation in Whole Blood of Patients with Clostridioides difficile Infection
International Journal of Molecular Sciences, 25 (23).
p. 12653.
ISSN 1661-6596, 1422-0067
Abstract
Clostridioides difficile (C. difficile) is a global threat and has significant implications for individuals and health care systems. Little is known about host molecular mechanisms and transcriptional changes in peripheral immune cells. This is the first gene expression study in whole blood from patients with C. difficile infection. We took blood and stool samples from patients with toxigenic C. difficile infection (CDI), non-toxigenic C. difficile infection (GDH), inflammatory bowel disease (IBD), diarrhea from other causes (DC), and healthy controls (HC). We performed transcriptome-wide RNA profiling on peripheral blood to identify diarrhea common and CDI unique gene sets. Diarrhea groups upregulated innate immune responses with neutrophils at the epicenter. The common signature associated with diarrhea was non-specific and shared by various other inflammatory conditions. CDI had a unique 45 gene set reflecting the downregulation of humoral and T cell memory functions. Dysregulation of immunometabolic genes was also abundant and linked to immune cell fate during differentiation. Whole transcriptome analysis of white cells in blood from patients with toxigenic C. difficile infection showed that there is an impairment of adaptive immunity and immunometabolism.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | <italic>Clostridioides difficile</italic>, transcriptomics, blood, adaptive immunity, immunometabolism |
| Divisions: | Faculty of Health & Life Sciences Faculty of Health & Life Sciences > Clinical Directorate Faculty of Health & Life Sciences > Inst. Systems, Molec & Integrative Biology > Inst. Systems, Molec & Integrative Biology Faculty of Health & Life Sciences > Tech, Infrastructure & Env Directorate |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 13 Dec 2024 15:47 |
| Last Modified: | 16 Jun 2026 17:10 |
| DOI: | 10.3390/ijms252312653 |
| Open Access URL: | https://doi.org/10.3390/ijms252312653 |
| Related Websites: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3189171 |
| Disclaimer: | The University of Liverpool is not responsible for content contained on other websites from links within repository metadata. Please contact us if you notice anything that appears incorrect or inappropriate. |
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