A 5-transcript signature for discriminating viral and bacterial etiology in pediatric pneumonia

Collapse authors list.
Viz-Lasheras, Sandra, Gómez-Carballa, Alberto, Pardo-Seco, Jacobo, Bello, Xabier, Rivero-Calle, Irene, Dacosta, Ana Isabel, Kaforou, Myrsini, Habgood-Coote, Dominic, Cunnington, Aubrey J, Emonts, Marieke et al (show 31 more authors) , Herberg, Jethro A, Wright, Victoria J, Carrol, Enitan D ORCID: 0000-0001-8357-7726, Paulus, Stephane C, Zenz, Werner, Kohlfürst, Daniela S, Van der Flier, Michiel, de Groot, Ronald, Schlapbach, Luregn J, Agyeman, Philipp, Pollard, Andrew J, Fink, Colin, Kuijpers, Taco T, Anderson, Suzanne, Calvo, Cristina, del Carmen Martínez-Padilla, María, Pérez-Aragón, Ana, Gómez-Sánchez, Esteban, Valencia-Ramos, Juan, Giménez-Sánchez, Francisco, Alonso-Quintela, Paula, Moreno-Galarraga, Laura, von Both, Ulrich, Pokorn, Marko, Zavadska, Dace, Tsolia, María, Vermont, Clementien L, Moll, Henriëtte A, Levin, Michael, Martinón-Torres, Federico and Salas, Antonio (2025) A 5-transcript signature for discriminating viral and bacterial etiology in pediatric pneumonia iScience, 28 (2). p. 111747. ISSN 2589-0042, 2589-0042

Text PIIS2589004225000069.pdf - Author Accepted Manuscript Available under License Creative Commons Attribution. Download (9MB) | Preview

Abstract

Pneumonia stands as the primary cause of death among children under five, yet current diagnosis methods often result in inadequate or unnecessary treatments. Our research seeks to address this gap by identifying host transcriptomic biomarkers in the blood of children with definitive viral and bacterial pneumonia. We performed RNA sequencing on 192 prospectively collected whole blood samples, including 38 controls and 154 pneumonia cases, uncovering a 5-transcript signature (genes FAM20A, BAG3, TDRD9, MXRA7, and KLF14) that effectively distinguishes bacterial from viral pneumonia (area under the curve (AUC): 0.95 [0.88–1.00]). Initial validation using combined definitive and probable cases yielded an AUC of 0.87 [0.77–0.97], while full validation in a new prospective cohort of 32 patients achieved an AUC of 0.92 [0.83–1.00]. This robust signature holds significant potential to enhance diagnostics accuracy for pediatric pneumonia, reducing diagnostic delays and unnecessary treatments and potentially transforming clinical practice.

Item Type:	Article
Uncontrolled Keywords:	EUCLIDS, DIAMONDS, GENDRES and, PERFORM consortia
Divisions:	Faculty of Health & Life Sciences Faculty of Health & Life Sciences > Inst. Infection, Vet & Ecological Sciences
Depositing User:	Symplectic Admin
Date Deposited:	08 Jan 2025 11:15
Last Modified:	28 Feb 2026 20:48
DOI:	10.1016/j.isci.2025.111747
Related Websites:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3189565
Disclaimer:	The University of Liverpool is not responsible for content contained on other websites from links within repository metadata. Please contact us if you notice anything that appears incorrect or inappropriate.