Nanoparticles may influence mast cells gene expression profiles without affecting their degranulation function

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Newton, HS, Cedrone, E, Grunberger, J, Xie, S, Zhao, Y, Tran, B, Toms, BS, Xu, W, Plant-Hately, A ORCID: 0000-0003-2425-1990, Liptrott, NJ ORCID: 0000-0002-5980-8966 et al (show 1 more authors) and Dobrovolskaia, MA (2025) Nanoparticles may influence mast cells gene expression profiles without affecting their degranulation function Nanomedicine Nanotechnology Biology and Medicine, 66. 102818-. ISSN 1549-9634, 1549-9642

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Abstract

An in vitro method for monitoring nanoparticle effects on IgE-dependent mast cell degranulation was developed and validated. The assayed nanoparticles included four clinical-grade nanomedicines (Abraxane, Doxil, AmBisome, and Feraheme) and three commercial research-grade nanomaterials (generation 5 PAMAM dendrimers with carboxy-, hydroxy-, or amine- surface functionalities). Most of the tested materials did not alter IgE-dependent mast cell degranulation, suggesting that nanoparticles and nanomedicines are unlikely to worsen pre-existing allergies to other antigens. Two clinical-grade formulations containing cytotoxic oncology drugs—Abraxane and Doxil—decreased degranulation. Abraxane but not Doxil decreased FcεR expression on the cell surface. Single-cell sequencing revealed the most differentially expressed genes (DEG) in Abraxane and Doxil-treated cultures. Interestingly, Feraheme and amine-terminated dendrimers induced DEG without affecting degranulation. These data demonstrate that some nanomaterials have more effects on immune cells than can be detected by a functional immunoassay.

Item Type:	Article
Uncontrolled Keywords:	Mast Cells, Animals, Humans, Immunoglobulin E, Receptors, IgE, Cell Degranulation, Dendrimers, Nanoparticles, Transcriptome
Divisions:	Faculty of Health & Life Sciences Faculty of Health & Life Sciences > Inst. Systems, Molec & Integrative Biology > Inst. Systems, Molec & Integrative Biology
Depositing User:	Symplectic Admin
Date Deposited:	23 Apr 2025 08:29
Last Modified:	22 Jan 2026 10:43
DOI:	10.1016/j.nano.2025.102818
Related Websites:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3191370
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