Maher, Rosemary E, Cytlak-Chaudhuri, Urszula M 
ORCID: 0000-0002-2536-6012, Aleem, Saad ORCID: 0009-0009-1196-3818, Barry, Peter ORCID: 0000-0001-6326-4431, Brice, Daniel Paul ORCID: 0000-0002-2507-3965, Caamaño Gutiérrez, Eva ORCID: 0000-0001-7737-5941, Driver, Kimberley ORCID: 0009-0001-1628-8331, Emmott, Edward ORCID: 0000-0002-3239-8178, Rothwell, Alexander ORCID: 0000-0001-5690-9481, Smith, Emily ORCID: 0009-0000-4261-6821 et al (show 7 more authors)
(2025)
Effect of elexacaftor/tezacaftor/ivacaftor on systemic inflammation in cystic fibrosis.
Thorax, 80 (9).
pp. 604-615.
ISSN 0040-6376, 1468-3296
Abstract
<h4>Background</h4>Despite significant clinical improvements, there is evidence of persisting airway inflammation in people with cystic fibrosis (CF) established on elexacaftor/tezacaftor/ivacaftor (ETI) therapy. As CF is a multi-system disease, systemic immune profiles can reflect local inflammation within the lungs and other organs. Understanding systemic inflammation after ETI therapy may reveal important translational insights. This study aims to profile systemic inflammatory changes and relate these to the well-documented improvements observed with ETI therapy.<h4>Methods</h4>We conducted a single-centre longitudinal study with 57 CF subjects initiating ETI therapy. All participants were Phe508del homozygous or Phe508del/minimal function. Blood samples were collected pre-ETI and 3-12 months post-therapy initiation. Analyses included mass spectrometry-based proteomics, a multiplex immunoassay, and flow cytometry for peripheral immune cell counts and phenotype. Controls samples were provided by 29 age-matched healthy controls.<h4>Results</h4>Systemic inflammation reduced with ETI therapy; however, the immune profile remained distinct from healthy controls. ETI reduced neutrophil counts and was associated with a more mature, less inflammatory phenotype, as well as a shift towards an immune resolving state associated with increased CD206 expression. Cytokines known to influence neutrophil levels reduced with therapy. Despite ETI therapy, neutrophil and monocyte counts remained elevated compared with healthy controls. There was no obvious association between the ETI-related improvements in systemic inflammation and lung function.<h4>Conclusions</h4>Patients with CF showed evidence of persisting systemic inflammation despite ETI therapy, which may have long-term potentially adverse effects on respiratory and other organ systems.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Humans, Cystic Fibrosis, Inflammation, Aminophenols, Pyrazoles, Pyridines, Pyrrolidines, Indoles, Quinolines, Quinolones, Drug Combinations, Longitudinal Studies, Adolescent, Adult, Child, Female, Male, Benzodioxoles, Young Adult, Chloride Channel Agonists |
| Divisions: | Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Life Courses and Medical Sciences Faculty of Health and Life Sciences > Institute of Systems, Molecular and Integrative Biology Faculty of Health and Life Sciences > Tech, Infrastructure and Environmental Directorate |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 20 Jun 2025 08:47 |
| Last Modified: | 23 Sep 2025 11:32 |
| DOI: | 10.1136/thorax-2024-222242 |
| Open Access URL: | https://thorax.bmj.com/content/early/2025/05/29/th... |
| Related Websites: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3193339 |
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