Williams, Christopher T, Romero-Ramirez, Alessandra, Semiu, Adeleye Adesola, Ifabumuyi, Samuel Oluwafunmbi, Greenland-Bews, Caitlin, Gould, Susan, Wooding, Dominic, Allen, Collette, Somasundaran, Anushri, Mkpuma, Nicodemus Nnabuike et al (show 16 more authors)
(2026)
Multiplex PCR to Differentiate Virus Clades
EMERGING INFECTIOUS DISEASES, 32 (2).
pp. 250-254.
ISSN 1080-6040, 1080-6059
Abstract
We designed a multiplex quantitative PCR to differentiate monkeypox virus clades. For clinical samples collected in the United Kingdom and Nigeria, sensitivity was 78% (95% CI 67.67%–86.14%) and specificity 94% (95% CI 80.84%–99.30%); for samples with cycle thresholds <35, sensitivity was 98% (95% CI 91.72%–99.96%) and specificity 94% (95% CI 80.84%–99.30%).
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | International Severe Acute Respiratory and Emerging Infection Consortium Investigators, Humans, Monkeypox virus, Sensitivity and Specificity, Phylogeny, Nigeria, Multiplex Polymerase Chain Reaction, United Kingdom, Mpox, Monkeypox |
| Divisions: | Faculty of Health & Life Sciences Faculty of Health & Life Sciences > Inst. Infection, Vet & Ecological Sciences Faculty of Health & Life Sciences > Inst. Infection, Vet & Ecological Sciences > Inst. Infection, Vet & Ecological Sciences (T&R Staff) Faculty of Health & Life Sciences > Inst. Infection, Vet & Ecological Sciences > Clinical Infection, Microbiology & Immunology |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 06 May 2026 14:51 |
| Last Modified: | 24 Aug 2026 15:04 |
| DOI: | 10.3201/eid3202.250686 |
| Open Access URL: | https://doi.org/10.3201%2Feid3202.250686 |
| Related Websites: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3198325 |
| Disclaimer: | The University of Liverpool is not responsible for content contained on other websites from links within repository metadata. Please contact us if you notice anything that appears incorrect or inappropriate. |
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