Susceptibility of microcosm subgingival dental plaques to lethal photosensitization

Allan, IU, O'Neill, JF and Hope, CK (2007) Susceptibility of microcosm subgingival dental plaques to lethal photosensitization. Biofilms. pp. 1-7.

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Abstract

Photodynamic therapy (PDT) offers potential as a non-invasive treatment of periodontal 15 disease. In this study, microcosm biofilms were grown in vitro under conditions designed 16 to mimic subgingival plaques typically found in patients with periodontitis. To 17 investigate potential PDT modalities, biofilms were exposed to light from a helium/neon 18 laser in conjunction with a photosensitiser, toluidine blue (TBO), at varying output and 19 concentration, respectively. To determine cytotoxic effects, viability profiling was 20 undertaken on whole biofilms using standard plating methods, and on horizontal cross-21 sections of biofilms using confocal laser-scanning microscopy (CLSM) in conjunction 22 with a differential viability stain. A light energy dose of 94.5 J in combination with 81.7 23 .M TBO was found to be optimal, achieving significant kills of over 97%. CLSM 24 enabled visualisation of the effects of PDT in three dimensions. Viability profiling of the 25 CLSM images revealed that lethal photosensitisation was most effective in the upper 26 layers of biofilm. PDT was found to reduce the viability of subgingivally-modelled 27 plaques in vitro by a similar magnitude to chlorhexidine digluconate (CHL), which is 28 commonly used to treat periodontal disease. The findings of this study indicate that PDT 29 may be an effective alternative to conventional modalities in the treatment of periodontal 30 disease. 31

Item Type:	Article
Additional Information:	7 pages. Citation: I. U. Allan, J. F. O'Neill and C. K. Hope Susceptibility of microcosm subgingival dental plaques to lethal photosensitization. Biofilms, Published online by Cambridge University Press 17 Dec 2007 doi:10.1017/S1479050507002219
Uncontrolled Keywords:	Lethal photosensitisation, Subgingival plaque, Oral Biofilm, Constant-depth film fermenter, Photodynamic therapy
Subjects:	?? RK ??
Divisions:	Faculty of Health and Life Sciences > Institute of Life Courses and Medical Sciences > School of Dentistry
Depositing User:	Symplectic Admin
Date Deposited:	20 Aug 2008 09:04
Last Modified:	17 Dec 2022 01:14
DOI:	10.1017/s1479050507002219
Publisher's Statement :	Copyright © 2007 Cambridge University Press. The paper has been accepted for publication and will appear in a revised form, subsequent to editorial input by Cambridge University Press, in the journal, Biofilms, I. U. Allan, J. F. O'Neill and C. K. Hope Susceptibility of microcosm subgingival dental plaques to lethal photosensitization. Biofilms, Published online by Cambridge University Press 17 Dec 2007. doi:10.1017/S1479050507002219 http://journals.cambridge.org/action/displayJournal?jid=BFM
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URI:	https://livrepository.liverpool.ac.uk/id/eprint/580