Investigation of strategies to protect against harmful bacteria-mucosa interaction in Crohn's disease and other diarrhoeal diseases

Knight, Paul (2011) Investigation of strategies to protect against harmful bacteria-mucosa interaction in Crohn's disease and other diarrhoeal diseases Doctor of Philosophy thesis, University of Liverpool.

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Abstract

The presence and replication of E.coli within Crohn’s Disease (CD) tissue has been confirmed by multiple authors and is hypothesised to be pivotal in the development of CD. In this thesis, quantification of E.coli bacteria within endoscopic biopsies has been achieved with high efficiency, sensitivity and reproducibility using PCR plasmid technology, and the technique’s utility demonstrated by quantifying E.coli within the biopsies of CD patients in clinical relapse and remission as well as within the first lesions present in CD relapse, aphthous ulcers. These studies showed some interesting correlations with clinical, macroscopic, and histological data, obtained during a clinical trial of soluble plantain fibre supplementation for prevention of relapse in CD. These included increased quantities of E.coli in tissues from the mucosa of patients in clinical relapse whose ileum was macroscopically normal yet histologically inflamed, and significant falls in the quantities of E.coli over time in the biopsy tissues of patients who were in clinical remission. The ability of a specific group of E.coli, the adherent invasive E. coli (AIEC), to replicate within macrophages, is increasingly perceived to be fundamental ... (continues)

Item Type:	Thesis (Doctor of Philosophy)
Additional Information:	Date: 2011-12 (completed)
Uncontrolled Keywords:	Crohn's macrophages plantain diarrhoea PCR
Divisions:	Faculty of Health & Life Sciences > Inst. Systems, Molec & Integrative Biology > Inst. Systems, Molec & Integrative Biology
Depositing User:	Symplectic Admin
Date Deposited:	10 Aug 2012 10:54
Last Modified:	31 Oct 2024 07:01
DOI:	10.17638/00005833
Supervisors:	Rhodes, Jon ORCID: 0000-0002-1302-260X Campbell, Barry ORCID: 0000-0002-7407-012X Winstanley, Craig ORCID: 0000-0002-2662-8053
URI:	https://livrepository.liverpool.ac.uk/id/eprint/5833
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