Investigation of the expression pattern and functional importance of the Gnasxl-encoded XLαs protein of the imprinted Gnas Locus



Burton, Katie
(2012) Investigation of the expression pattern and functional importance of the Gnasxl-encoded XLαs protein of the imprinted Gnas Locus. Doctor of Philosophy thesis, University of Liverpool.

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Abstract

XLαs is a NH2-terminal splice variant of the stimulatory G-protein α-subunit Gsα. Both are encoded by the imprinted Gnas locus. Similar to Gsα, XLαs can couple 7-TM receptors to adenylate cyclase in cultured cells. Previously, a knock-out mouse specific for the Gnasxl transcript was generated (Gnasxlm+/p-) (PLAGGE et al. 2004). This mouse model exhibits a lean, hypermetabolic phenotype with elevated sympathetic nervous system (SNS) activity (XIE et al. 2006). Changes in phenotype between neonatal and adult stages (e.g. mortality; failure to thrive as neonates vs. healthy but hypermetabolic adults) are not yet fully understood. More recently a conditional gene trap knock-out mouse line (XLlacZGT) was established. Immunohistochemistry, XGal and immunofluorescence data were collected to document the changes in expression pattern of XLαs and determine possible signalling pathways affected by lack of XLαs in the brain; histological analysis of the lacZ-containing genetrap line revealed new sites of XLαs expression. A comparison of neonatal and adult brain expression patterns revealed that the lateral hypothalamus (LH), dorsomedial hypothalamus (DMH), arcuate nucleus (Arc), locus coeruleus and ventrolateral ... (continues)

Item Type: Thesis (Doctor of Philosophy)
Additional Information: Date: 2012 (completed)
Divisions: Faculty of Health and Life Sciences > Institute of Systems, Molecular and Integrative Biology
Depositing User: Symplectic Admin
Date Deposited: 03 Aug 2012 09:41
Last Modified: 02 Nov 2024 11:36
DOI: 10.17638/00006213
Supervisors:
URI: https://livrepository.liverpool.ac.uk/id/eprint/6213