Use of suppression subtractive hybridisation to extend our knowledge of genome diversity in Campylobacter jejuni



Hepworth, Philip J, Leatherbarrow, Howard, Hart, C Anthony and Winstanley, Craig ORCID: 0000-0002-2662-8053
(2007) Use of suppression subtractive hybridisation to extend our knowledge of genome diversity in Campylobacter jejuni. BMC Genomics, 8 (1).

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Abstract

<jats:title>Abstract</jats:title> <jats:sec> <jats:title>Background</jats:title> <jats:p>Previous studies have sought to identify a link between the distribution of variable genes amongst isolates of <jats:italic>Campylobacter jejuni</jats:italic> and particular host preferences. The genomic sequence data available currently was obtained using only isolates from human or chicken hosts. In order to identify variable genes present in isolates from alternative host species, five subtractions between <jats:italic>C. jejuni</jats:italic> isolates from different sources (rabbit, cattle, wild bird) were carried out, designed to assess genomic variability within and between common multilocus sequence type (MLST) clonal complexes (ST-21, ST-42, ST-45 and ST-61).</jats:p> </jats:sec> <jats:sec> <jats:title>Results</jats:title> <jats:p>The vast majority (97%) of the 195 subtracted sequences identified had a best BLASTX match with a <jats:italic>Campylobacter</jats:italic> protein. However, there was considerable variation within and between the four clonal complexes included in the subtractions. The distributions of eight variable sequences, including four with putative roles in the use of alternative terminal electron acceptors, amongst a panel of <jats:italic>C. jejuni</jats:italic> isolates representing diverse sources and STs, were determined.</jats:p> </jats:sec> <jats:sec> <jats:title>Conclusion</jats:title> <jats:p>There was a clear correlation between clonal complex and the distribution of the metabolic genes. In contrast, there was no evidence to support the hypothesis that the distribution of such genes may be related to host preference. The other variable genes studied were also generally distributed according to MLST type. Thus, we found little evidence for widespread horizontal gene transfer between clonal complexes involving these genes.</jats:p> </jats:sec>

Item Type: Article
Subjects: ?? R1 ??
Divisions: Faculty of Health and Life Sciences
Faculty of Health and Life Sciences > Institute of Infection, Veterinary and Ecological Sciences > School of Veterinary Science
Depositing User: Symplectic Admin
Date Deposited: 25 Jun 2008 14:23
Last Modified: 26 Apr 2022 10:28
DOI: 10.1186/1471-2164-8-110
Publisher's Statement : © 2007 Hepworth et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
URI: https://livrepository.liverpool.ac.uk/id/eprint/703