A proteomics study of the response of North Ronaldsay sheep to copper challenge



Simpson, Deborah M, Mobasheri, Ali, Haywood, Susan and Beynon, Robert J
(2006) A proteomics study of the response of North Ronaldsay sheep to copper challenge. BMC Veterinary Research, 2.

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Abstract

Background: The objective of this proteomics study was to identify proteins that changed expression as a result of copper challenge in the uniquely copper sensitive North Ronaldsay sheep and further, to compare those changes in expression with the more copper tolerant Cambridge breed. Such data gives us a proteome-centered perspective of the pathogenesis of copper-induced oxidative stress in this breed. Results: Many proteins respond to copper challenge, but this study focuses on those exhibiting a differential response between the two breeds, related to liver copper content. As copper accumulated in the tissue, the pattern of expression of several proteins was markedly different, in North Ronaldsay sheep as compared to the Cambridge breed. Conclusion: The pattern of changes was consistent with the greatly enhanced susceptibility of North Ronaldsay sheep to copper-induced oxidative stress, focused on mitochondrial disturbance with consequent activation of hepatic stellate cells. The expression profiles were sufficiently complex that the response could not simply be explained as a hypersensitivity to copper in North Ronaldsay sheep.

Item Type: Article
Additional Information: Published: 27 December 2006. 14 pages (page numbers not for citation purposes).
Uncontrolled Keywords: Cambridge sheep, pathogenesis, copper-induced oxidative stress, hepatic stellate cells
Subjects: S Agriculture > SF Animal culture
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Depositing User: Symplectic Admin
Date Deposited: 27 Jun 2008 15:00
Last Modified: 09 Jan 2021 08:59
DOI: 10.1186/1746-6148-2-36
Publisher's Statement : © 2006 Simpson et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
URI: https://livrepository.liverpool.ac.uk/id/eprint/750