Analysis of normal and osteoarthritic canine cartilage mRNA expression by quantitative polymerase chain reaction

Clements, Dylan N, Carter, Stuart D ORCID: 0000-0002-3585-9400, Innes, John F, Ollier, William ER and Day, Philip JR
(2006) Analysis of normal and osteoarthritic canine cartilage mRNA expression by quantitative polymerase chain reaction. ARTHRITIS RESEARCH & THERAPY, 8 (6). R158-.

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The molecular basis to mammalian osteoarthritis (OA) is unknown. We hypothesised that the expression of selected proteases, matrix molecules, and collagens believed to have a role in the pathogenesis of OA would be changed in naturally occurring canine OA cartilage when compared to normal articular cartilage. Quantitative (real-time) reverse transcriptase-polymerase chain reaction assays were designed measuring the expression of selected matrix molecules (collagens and small leucine-rich proteoglycans), key mediators of the proteolytic degradation of articular cartilage (metalloproteinases, cathepsins), and their inhibitors (tissue inhibitors of matrix metalloproteinases). All data were normalised using a geometric mean of three housekeeping genes, and the results subjected to power calculations and corrections for multiple hypothesis testing. We detected increases in the expression of BGN, COL1A2, COL2A1, COL3A1, COL5A1, CSPG2, CTSB, CTSD, LUM, MMP13, TIMP1, and TNC in naturally occurring canine OA. The expression of TIMP2 and TIMP4 was significantly reduced in canine OA cartilage. The patterns of gene expression change observed in naturally occurring canine OA were similar to those reported in naturally occurring human OA and experimental canine OA. We conclude that the expression profiles of matrix-associated molecules in end-stage mammalian OA may be comparable but that the precise aetiologies of OA affecting specific joints in different species are presently unknown.

Item Type: Article
Additional Information: Published: 10 Oct 2006. 9 pages (page numbers not for citation purposes).
Uncontrolled Keywords: Cartilage, Articular, Animals, Dogs, Humans, Osteoarthritis, Collagen, Cathepsins, Metalloproteases, Proteoglycans, Tissue Inhibitor of Metalloproteinases, RNA, Messenger, Reverse Transcriptase Polymerase Chain Reaction, Gene Expression
Subjects: ?? SF ??
Divisions: Faculty of Health and Life Sciences > Institute of Infection, Veterinary and Ecological Sciences > School of Veterinary Science
Depositing User: Symplectic Admin
Date Deposited: 01 Jul 2008 10:17
Last Modified: 15 Mar 2024 08:29
DOI: 10.1186/ar2053
Publisher's Statement : © 2006 Clements et al.; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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