Molecular staging of surgical margins in oral squamous cell carcinoma using promoter methylation of p16INK4A, cytoglobin, E-cadherin, and TMEFF2

Shaw, RJ ORCID: 0000-0001-7027-8997, Hobkirk, AJ, Nikolaidis, G, Woolgar, JA, Triantafyllou, A, Brown, JS, Liloglou, T ORCID: 0000-0003-0460-1404 and Risk, JM ORCID: 0000-0002-8770-7783 (2013) Molecular staging of surgical margins in oral squamous cell carcinoma using promoter methylation of p16INK4A, cytoglobin, E-cadherin, and TMEFF2 Annals of Surgical Oncology, 20 (8). pp. 2796-2802. ISSN 1068-9265, 1534-4681

	Text Molecular_staging_of_surgical_margins_in_oral_cancer_-_revised.docx - Author Accepted Manuscript Download (89kB)
	Text Supplementary_Table_corrected.xlsx - Unspecified Download (20kB)
	Text 7701.pdf - Author Accepted Manuscript Download (421kB)

Abstract

Background: Local recurrence in oral squamous cell carcinoma (OSCC) despite clear surgical margins may indicate the presence of residual, sub-microscopic disease. Molecular assessment of surgical margins may provide a greater prognostic sensitivity compared to histopathology. We aimed to determine whether promoter methylation in deep and mucosal resection margins can predict recurrence in OSCC. Methods: Forty-eight consecutive OSCC cases were recruited and a 5 mm³ tumor sample plus 5 deep and 5 mucosal margin samples were snap frozen. Clinical, pathological, adjuvant therapy, and outcome data were recorded. Tumors were informative if >5 % promoter methylation was found for ≥1 of 4 genes using qMSP. Margins were declared molecularly positive if >1 % promoter methylation was found in any margin. Results: Thirty (63 %) of 48 cases were methylation informative. Mucosal margin samples were largely positive for methylation (26 of 30, 87 %), indicating the presence of field cancerization. Methylation at ≥1 gene promoters in ≥1 deep margin correlated with the presence of close/involved mucosal margins (P = 0.027) and increased pT status (P = 0.027) but not the status of deep margins, recurrence, or survival. Conclusions: The current gene panel did not add prognostic information to histopathological reporting of resection margins. Future efforts should concentrate on improving gene selection, informativity, and assay performance in the patient group with intermediate indications for adjuvant therapy. © 2012 Society of Surgical Oncology.

Item Type:	Article
Additional Information:	Issue date: 1st August 2013. Available online 31 October 2012.
Uncontrolled Keywords:	Humans, Carcinoma, Squamous Cell, Mouth Neoplasms, Neoplasm Recurrence, Local, Neoplasm, Residual, Globins, Cadherins, Membrane Proteins, Neoplasm Proteins, DNA Methylation, Genes, p16, Aged, Middle Aged, Female, Male, Promoter Regions, Genetic, Kaplan-Meier Estimate, Cytoglobin
Subjects:	?? RK ??
Divisions:	Faculty of Health & Life Sciences > Inst. Systems, Molec & Integrative Biology > Inst. Systems, Molec & Integrative Biology
Depositing User:	Symplectic Admin
Date Deposited:	20 Nov 2012 16:28
Last Modified:	01 Mar 2026 12:08
DOI:	10.1245/s10434-012-2713-8
Publisher's Statement :	Originally published in Annals of Surgical Oncology. The final publication is available at The final publication is available at Springer via http://dx.doi.org/10.1245/s10434-012-2713-8.
Related Websites:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/7701
Disclaimer:	The University of Liverpool is not responsible for content contained on other websites from links within repository metadata. Please contact us if you notice anything that appears incorrect or inappropriate.