Molecular staging of surgical margins in oral squamous cell carcinoma using promoter methylation of p16(INK4A), cytoglobin, E-cadherin, and TMEFF2.



Shaw, Richard J ORCID: 0000-0002-5157-4042, Hobkirk, Andrew J, Nikolaidis, George, Woolgar, Julia A, Triantafyllou, Asterios, Brown, James S, Liloglou, Triantafillos ORCID: 0000-0003-0460-1404 and Risk, Janet M ORCID: 0000-0002-8770-7783
(2013) Molecular staging of surgical margins in oral squamous cell carcinoma using promoter methylation of p16(INK4A), cytoglobin, E-cadherin, and TMEFF2. Annals of surgical oncology, 20 (8). 2796 - 2802.

This is the latest version of this item.

[img] Text
Molecular_staging_of_surgical_margins_in_oral_cancer_-_revised.docx - Accepted Version

Download (89kB)
[img] Text
Supplementary_Table_corrected.xlsx - Unspecified

Download (20kB)
[img] Text
7701.pdf - Accepted Version

Download (421kB)

Abstract

<h4>Background</h4>Local recurrence in oral squamous cell carcinoma (OSCC) despite clear surgical margins may indicate the presence of residual, sub-microscopic disease. Molecular assessment of surgical margins may provide a greater prognostic sensitivity compared to histopathology. We aimed to determine whether promoter methylation in deep and mucosal resection margins can predict recurrence in OSCC.<h4>Methods</h4>Forty-eight consecutive OSCC cases were recruited and a 5 mm(3) tumor sample plus 5 deep and 5 mucosal margin samples were snap frozen. Clinical, pathological, adjuvant therapy, and outcome data were recorded. Tumors were informative if >5 % promoter methylation was found for ≥1 of 4 genes using qMSP. Margins were declared molecularly positive if >1 % promoter methylation was found in any margin.<h4>Results</h4>Thirty (63 %) of 48 cases were methylation informative. Mucosal margin samples were largely positive for methylation (26 of 30, 87 %), indicating the presence of field cancerization. Methylation at ≥1 gene promoters in ≥1 deep margin correlated with the presence of close/involved mucosal margins (P = 0.027) and increased pT status (P = 0.027) but not the status of deep margins, recurrence, or survival.<h4>Conclusions</h4>The current gene panel did not add prognostic information to histopathological reporting of resection margins. Future efforts should concentrate on improving gene selection, informativity, and assay performance in the patient group with intermediate indications for adjuvant therapy.

Item Type: Article
Uncontrolled Keywords: Humans, Carcinoma, Squamous Cell, Mouth Neoplasms, Neoplasm Recurrence, Local, Neoplasm, Residual, Globins, Cadherins, Membrane Proteins, Neoplasm Proteins, DNA Methylation, Genes, p16, Aged, Middle Aged, Female, Male, Promoter Regions, Genetic, Kaplan-Meier Estimate, Cytoglobin
Subjects: R Medicine > RK Dentistry
Divisions: ?? sch_cancer ??
Depositing User: Symplectic Admin
Date Deposited: 20 Nov 2012 16:28
Last Modified: 05 Mar 2021 08:10
DOI: 10.1245/s10434-012-2713-8
Publisher's Statement : Originally published in Annals of Surgical Oncology. The final publication is available at The final publication is available at Springer via http://dx.doi.org/10.1245/s10434-012-2713-8.
URI: https://livrepository.liverpool.ac.uk/id/eprint/7701

Available Versions of this Item

  • Molecular staging of surgical margins in oral squamous cell carcinoma using promoter methylation of p16(INK4A), cytoglobin, E-cadherin, and TMEFF2. (deposited 20 Nov 2012 16:28) [Currently Displayed]