Wray, Thomas
Developments in dynamic field gradient focusing: microfluidics and integration.
Doctor of Philosophy thesis, University of Liverpool.
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Abstract
Advances in modern science require the development of more robust and improved systems for electroseparations in chromatography. In response, the progress of a new analytical platform is discussed. DFGF (Dynamic Field Gradient Focusing) is a separation technique, first described in 1998, which exploits the differences in electrophoretic mobility and hydrodynamic area of analytes to result in separation. This is achieved by taking a channel and applying a hydrodynamic flow in one direction and a counteracting electric field gradient acting in the opposite direction, resulting in analytes reaching a focal point according to their electrophoretic mobility. Work through this project has seen innovations to improve existing DFGF devices, including the design and manufacture of a novel packing material, while developing the latest DFGF system. This incorporates a microfluidic separation channel, eliminating the need for packing material or monolith. The new microfluidic device also features whole-on-column UV detection. Improvements through the developments of this device are discussed, most notably the utilisation of a new rapid prototyping technique. Examples of applications undertaken with the new device are demonstrated including novel samples and integration with mass spectrometry and 2D-HPLC.
| Item Type: | Thesis (Doctor of Philosophy) |
|---|---|
| Additional Information: | Date: 2012-08 (completed) |
| Uncontrolled Keywords: | Dynamic Field Gradient Focusing, DFGF, Chromatography, Separation Science, Instrumentation, 2D-HPLC, Counter acting Chromatography, Electrophoretic Separation, Protein Separation, Electrophoresis, Microfluidics, Whole-on-column Detection |
| Subjects: | ?? Q1 ?? ?? QD ?? |
| Divisions: | Faculty of Science and Engineering > School of Physical Sciences |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 17 Jan 2013 12:50 |
| Last Modified: | 16 Dec 2022 04:36 |
| DOI: | 10.17638/00007973 |
| Supervisors: |
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| URI: | https://livrepository.liverpool.ac.uk/id/eprint/7973 |
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