Smalley, John W, Birss, Andrew J, Szmigielski, Borys and Potempa, Jan
(2008)
Mechanism of methaemoglobin breakdown by the lysine-specific gingipain of the periodontal pathogen Porphyromonas gingivalis.
Biological Chemistry, 389 (9).
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Abstract
<jats:title>Abstract</jats:title><jats:p>The R- and K-gingipain proteases of<jats:italic>Porphyromonas gingivalis</jats:italic>are involved in proteolysis of haemoglobin from which the defensive dimeric haem pigment is formed. Whilst oxyhaemoglobin is refractory towards K-gingipain, methaemoglobin is rapidly degraded. Ligation of methaemoglobin with N<jats:sub>3</jats:sub><jats:sup>-</jats:sup>, which effectively blocks haem dissociation from the protein, prevented haemoglobin breakdown. Haem-free globin was rapidly degraded by K-gingipain. These data emphasise the need for haemoglobin oxidation which encourages haem dissociation and makes the haem-free globin susceptible to proteolytic attack.</jats:p>
| Item Type: | Article |
|---|---|
| Subjects: | ?? RK ?? |
| Divisions: | Faculty of Health and Life Sciences > Institute of Life Courses and Medical Sciences > School of Dentistry |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 05 Dec 2008 10:17 |
| Last Modified: | 02 Apr 2021 07:11 |
| DOI: | 10.1515/bc.2008.140 |
| Publisher's Statement : | The Version of Record (VoR) is available at www.biochemj.org |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/946 |
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