Mechanism of methaemoglobin breakdown by the lysine-specific gingipain of the periodontal pathogen Porphyromonas gingivalis



Smalley, John W, Birss, Andrew J, Szmigielski, Borys and Potempa, Jan
(2008) Mechanism of methaemoglobin breakdown by the lysine-specific gingipain of the periodontal pathogen Porphyromonas gingivalis. Biological Chemistry, 389 (9).

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Abstract

<jats:title>Abstract</jats:title><jats:p>The R- and K-gingipain proteases of<jats:italic>Porphyromonas gingivalis</jats:italic>are involved in proteolysis of haemoglobin from which the defensive dimeric haem pigment is formed. Whilst oxyhaemoglobin is refractory towards K-gingipain, methaemoglobin is rapidly degraded. Ligation of methaemoglobin with N<jats:sub>3</jats:sub><jats:sup>-</jats:sup>, which effectively blocks haem dissociation from the protein, prevented haemoglobin breakdown. Haem-free globin was rapidly degraded by K-gingipain. These data emphasise the need for haemoglobin oxidation which encourages haem dissociation and makes the haem-free globin susceptible to proteolytic attack.</jats:p>

Item Type: Article
Subjects: R Medicine > RK Dentistry
Divisions: ?? sch_dental ??
Depositing User: Symplectic Admin
Date Deposited: 05 Dec 2008 10:17
Last Modified: 09 Jan 2021 08:59
DOI: 10.1515/bc.2008.140
Publisher's Statement : The Version of Record (VoR) is available at www.biochemj.org
URI: https://livrepository.liverpool.ac.uk/id/eprint/946