Application of next-generation sequencing technologies in virology



Radford, Alan D ORCID: 0000-0002-4590-1334, Chapman, David, Dixon, Linda, Chantrey, Julian ORCID: 0000-0002-4801-7034, Darby, Alistair C ORCID: 0000-0002-3786-6209 and Hall, Neil
(2012) Application of next-generation sequencing technologies in virology. JOURNAL OF GENERAL VIROLOGY, 93 (Pt 9). pp. 1853-1868.

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Abstract

The progress of science is punctuated by the advent of revolutionary technologies that provide new ways and scales to formulate scientific questions and advance knowledge. Following on from electron microscopy, cell culture and PCR, next-generation sequencing is one of these methodologies that is now changing the way that we understand viruses, particularly in the areas of genome sequencing, evolution, ecology, discovery and transcriptomics. Possibilities for these methodologies are only limited by our scientific imagination and, to some extent, by their cost, which has restricted their use to relatively small numbers of samples. Challenges remain, including the storage and analysis of the large amounts of data generated. As the chemistries employed mature, costs will decrease. In addition, improved methods for analysis will become available, opening yet further applications in virology including routine diagnostic work on individuals, and new understanding of the interaction between viral and host transcriptomes. An exciting era of viral exploration has begun, and will set us new challenges to understand the role of newly discovered viral diversity in both disease and health.

Item Type: Article
Additional Information: ## TULIP Type: Articles/Papers (Journal) ##
Uncontrolled Keywords: Animals, Humans, Viruses, Sequence Analysis, DNA, Virology, High-Throughput Nucleotide Sequencing
Depositing User: Symplectic Admin
Date Deposited: 16 Feb 2015 16:04
Last Modified: 16 Dec 2022 04:43
DOI: 10.1099/vir.0.043182-0
Publisher's Statement : This is not the version of record of this article. This is an author accepted manuscript (AAM) that has been accepted for publication in Journal of General Virology that has not been copy-edited, typeset or proofed. The Society for General Microbiology (SGM) does not permit the posting of AAMs for commercial use or systematic distribution. SGM disclaims any responsibility or liability for errors or omissions in this version of the manuscript or in any version derived from it by any other parties. The final version is available at http://dx.doi.org/ 10.1099/vir.0.043182-0, 2012.
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URI: https://livrepository.liverpool.ac.uk/id/eprint/2007010