Liu, Xiaohong, Li, Jiasong ORCID: 0000-0003-0341-2038, Dong, Jianshu ORCID: 0000-0002-4119-8039, Hu, Cheng, Gong, Weimin and Wang, Jiangyun
(2012)
Genetic incorporation of a metal-chelating amino acid as a probe for protein electron transfer.
Angewandte Chemie (International ed. in English), 51 (41).
pp. 10261-10265.
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Abstract
ET encounters jellyfish: Through the incorporation of the metal-chelating amino acid pyTyr into green fluorescent protein (GFP) from jellyfish, photoinduced electron transfer (ET) from the GFP chromophore to a bound Cu II ion was shown to occur within one nanosecond in a distance-dependent manner. The crystal structure of GFP with pyTyr at a specific position shows the structural basis for the nanomolar binding affinity of pyTyr to CuII ions. Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Item Type: | Article |
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Uncontrolled Keywords: | Animals, Scyphozoa, Metals, Amino Acids, Tyrosine, Green Fluorescent Proteins, Chelating Agents, Protein Engineering, Binding Sites, Protein Structure, Tertiary, Electron Transport |
Subjects: | ?? QD ?? |
Depositing User: | Symplectic Admin |
Date Deposited: | 26 Nov 2015 16:27 |
Last Modified: | 17 Dec 2022 01:33 |
DOI: | 10.1002/anie.201204962 |
Related URLs: | |
URI: | https://livrepository.liverpool.ac.uk/id/eprint/2039304 |