The Extracellular Vesicles of the Helminth Pathogen, <i>Fasciola hepatica</i>: Biogenesis Pathways and Cargo Molecules Involved in Parasite Pathogenesis



Cwiklinski, Krystyna ORCID: 0000-0001-5577-2735, de la Torre-Escudero, Eduardo, Trelis, Maria, Bernal, Dolores, Dufresne, Philippe J, Brennan, Gerard P, O'Neill, Sandra, Tort, Jose, Paterson, Steve ORCID: 0000-0002-1307-2981, Marcilla, Antonio
et al (show 2 more authors) (2015) The Extracellular Vesicles of the Helminth Pathogen, <i>Fasciola hepatica</i>: Biogenesis Pathways and Cargo Molecules Involved in Parasite Pathogenesis. MOLECULAR & CELLULAR PROTEOMICS, 14 (12). pp. 3258-3273.

[thumbnail of Molecular & Cellular Proteomics 2015 Cwiklinski.pdf] Text
Molecular & Cellular Proteomics 2015 Cwiklinski.pdf - Unspecified

Download (2MB)
[thumbnail of admin] Atom XML (admin)
2017-05-12T18:46:08Z.atom - Unspecified

Download (0B)
[thumbnail of admin] Atom XML (admin)
2017-05-12T19:47:10Z.atom - Unspecified

Download (0B)
[thumbnail of admin] Atom XML (admin)
2017-05-12T20:46:11Z.atom - Unspecified

Download (0B)
[thumbnail of admin] Atom XML (admin)
2017-05-12T21:46:19Z.atom - Unspecified

Download (0B)
[thumbnail of admin] Atom XML (admin)
2017-05-12T22:47:42Z.atom - Unspecified

Download (0B)
[thumbnail of admin] Atom XML (admin)
2017-05-12T23:54:23Z.atom - Unspecified

Download (0B)
[thumbnail of admin] Atom XML (admin)
2017-05-13T01:49:05Z.atom - Unspecified

Download (0B)
[thumbnail of admin] Atom XML (admin)
2017-05-13T02:47:34Z.atom - Unspecified

Download (0B)
[thumbnail of admin] Atom XML (admin)
2017-05-13T03:47:39Z.atom - Unspecified

Download (0B)
[thumbnail of admin] Atom XML (admin)
2017-05-13T05:12:02Z.atom - Unspecified

Download (0B)
[thumbnail of admin] Atom XML (admin)
2017-05-13T05:47:50Z.atom - Unspecified

Download (0B)
[thumbnail of admin] Atom XML (admin)
2017-05-13T06:46:37Z.atom - Unspecified

Download (0B)
[thumbnail of admin] Atom XML (admin)
2017-05-13T07:47:05Z.atom - Unspecified

Download (0B)
[thumbnail of admin] Atom XML (admin)
2017-05-13T08:47:52Z.atom - Unspecified

Download (0B)
[thumbnail of admin] Atom XML (admin)
2017-05-13T09:46:42Z.atom - Unspecified

Download (0B)

Abstract

Extracellular vesicles (EVs) released by parasites have important roles in establishing and maintaining infection. Analysis of the soluble and vesicular secretions of adult Fasciola hepatica has established a definitive characterization of the total secretome of this zoonotic parasite. Fasciola secretes at least two subpopulations of EVs that differ according to size, cargo molecules and site of release from the parasite. The larger EVs are released from the specialized cells that line the parasite gastrodermus and contain the zymogen of the 37 kDa cathepsin L peptidase that performs a digestive function. The smaller exosome-like vesicle population originate from multivesicular bodies within the tegumental syncytium and carry many previously described immunomodulatory molecules that could be delivered into host cells. By integrating our proteomics data with recently available transcriptomic data sets we have detailed the pathways involved with EV biogenesis in F. hepatica and propose that the small exosome biogenesis occurs via ESCRT-dependent MVB formation in the tegumental syncytium before being shed from the apical plasma membrane. Furthermore, we found that the molecular "machinery" required for EV biogenesis is constitutively expressed across the intramammalian development stages of the parasite. By contrast, the cargo molecules packaged within the EVs are developmentally regulated, most likely to facilitate the parasites migration through host tissue and to counteract host immune attack.

Item Type: Article
Uncontrolled Keywords: Animals, Fasciola hepatica, Helminth Proteins, Gene Expression Profiling, Proteomics, Gene Expression Regulation, Developmental, Extracellular Vesicles
Depositing User: Symplectic Admin
Date Deposited: 04 Dec 2015 11:35
Last Modified: 17 Oct 2023 01:01
DOI: 10.1074/mcp.M115.053934
Publisher's Statement : Creative commons OA
Related URLs:
URI: https://livrepository.liverpool.ac.uk/id/eprint/2041183