A critical role of T follicular helper cells in human mucosal anti-influenza response that can be enhanced by immunological adjuvant CpG-DNA



Aljurayyan, AN, Sharma, R, Upile, N, Beer, H, Vaughan, C, Xie, C, Achar, P, Ahmed, MS ORCID: 0000-0001-6163-8102, McNamara, PS ORCID: 0000-0002-7055-6034, Gordon, SB
et al (show 1 more authors) (2016) A critical role of T follicular helper cells in human mucosal anti-influenza response that can be enhanced by immunological adjuvant CpG-DNA. ANTIVIRAL RESEARCH, 132. pp. 122-130.

This is the latest version of this item.

[img] Text
A critical role of T follicular helper cells in human mucosal anti-influenza response.pdf - Author Accepted Manuscript

Download (1MB)

Abstract

T Follicular helper cells (TFH) are considered critical for B cell antibody response, and recent efforts have focused on promoting TFH in order to enhance vaccine efficacy. We studied the frequency and function of TFH in nasopharynx-associated lymphoid tissues (NALT) from children and adults, and its role in anti-influenza antibody response following stimulation by a live-attenuated influenza vaccine (LAIV) or an inactivated seasonal virus antigen (sH1N1). We further studied whether CpG-DNA promotes TFH and by which enhances anti-influenza response. We showed NALT from children aged 1.5-10 years contained abundant TFH, suggesting efficient priming of TFH during early childhood. Stimulation by LAIV induced a marked increase in TFH that correlated with a strong production of anti-hemagglutinin (HA) IgA/IgG/IgM antibodies in tonsillar cells. Stimulation by the inactivated sH1N1 antigen induced a small increase in TFH which was markedly enhanced by CpG-DNA, accompanied by enhanced anti-HA antibody responses. In B cell co-culture experiment, anti-HA responses were only seen in the presence of TFH, and addition of plasmacytoid dendritic cell to TFH-B cell co-culture enhanced the TFH-mediated antibody production following CpG-DNA and sH1N1 antigen stimulation. Induction of TFH differentiation from naïve T cells was also shown following the stimulation. Our results support a critical role of TFH in human mucosal anti-influenza antibody response. Use of an adjuvant such as CpG-DNA that has the capacity to promote TFH by which to enhance antigen-induced antibody responses in NALT tissue may have important implications for future vaccination strategies against respiratory pathogens.

Item Type: Article
Uncontrolled Keywords: T follicular helper cell (T-FH), Influenza virus, Influenza vaccine, Anti-hemagglutinin (HA) antibody response, Nasopharynx-associated lymphoid tissues (NALT), CpG-DNA, Children and adults
Depositing User: Symplectic Admin
Date Deposited: 11 Jan 2017 10:47
Last Modified: 19 Jan 2023 07:24
DOI: 10.1016/j.antiviral.2016.05.021
Related URLs:
URI: https://livrepository.liverpool.ac.uk/id/eprint/3004695

Available Versions of this Item