Serodiagnosis of Mycobacterium abscessus complex infection in cystic fibrosis

Qvist, T, Pressler, T, Taylor-Robinson, David ORCID: 0000-0002-5828-7724, Katzenstein, TL and Hoiby, N (2015) Serodiagnosis of Mycobacterium abscessus complex infection in cystic fibrosis. European Respiratory Journal, 46 (3). pp. 707-716.

Text qvist_2015.pdf - Submitted version Download (722kB)

Abstract

Early signs of pulmonary disease with Mycobacterium abscessus complex (MABSC) can be missed in patients with cystic fibrosis (CF). A serological method could help stratify patients according to risk. The objective of this study was to test the diagnostic accuracy of a novel method for investigating IgG activity against MABSC. A prospective study of all patients attending the Copenhagen CF Centre was conducted by culturing for MABSC during a 22-month period and then screening patients with an anti-MABSC IgG ELISA. Culture-positive patients had stored serum examined for antibody kinetics before and after culture conversion. 307 patients had 3480 respiratory samples cultured and were then tested with the anti-MABSC IgG ELISA. Patients with MABSC pulmonary disease had median anti-MABSC IgG levels six-fold higher than patients with no history of infection (434 versus 64 ELISA units; p<0.001). The test sensitivity was 95% (95% CI 74–99%) and the specificity was 73% (95% CI 67–78%). A diagnostic algorithm was constructed to stratify patients according to risk. The test accurately identified patients with pulmonary disease caused by MABSC and was suited to be used as a complement to mycobacterial culture.

Item Type:	Article
Uncontrolled Keywords:	Humans, Cystic Fibrosis, Enzyme-Linked Immunosorbent Assay, Serologic Tests, Severity of Illness Index, Prevalence, Multivariate Analysis, Risk Assessment, Case-Control Studies, Longitudinal Studies, Predictive Value of Tests, Needs Assessment, Adolescent, Adult, Child, Child, Preschool, Denmark, Female, Male, Young Adult, Mycobacterium Infections, Nontuberculous, Nontuberculous Mycobacteria
Depositing User:	Symplectic Admin
Date Deposited:	08 Feb 2018 14:22
Last Modified:	19 Jan 2023 06:42
DOI:	10.1183/09031936.00011815
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3017649