Photoresponsive Hydrogels with Photoswitchable Mechanical Properties Allow Time-Resolved Analysis of Cellular Responses to Matrix Stiffening



Lee, I-NIng, Dobres, Oana, Richards, David, Ballestrem, Christoph, Curran, JM ORCID: 0000-0003-1551-2917, Hunt, John ORCID: 0000-0002-5168-4778, Richardson, Stephen, Swift, Joe and Wong, Lu Shin
(2018) Photoresponsive Hydrogels with Photoswitchable Mechanical Properties Allow Time-Resolved Analysis of Cellular Responses to Matrix Stiffening. ACS Applied Materials and Interfaces, 10 (9). pp. 7765-7776.

[img] Text
L22A Light tuneable substrates ACS Applied Materials & Interfaces v11 wi....pdf - Author Accepted Manuscript

Download (1MB)
[img] Text
L22A SI v9.pdf - Author Accepted Manuscript

Download (914kB)

Abstract

As cell function and phenotype can be directed by the mechanical characteristics of the surrounding matrix, hydrogels have become important platforms for cell culture systems, with properties that can be tuned by external stimuli, such as divalent cations, enzymatic treatment, and pH. However, many of these stimuli can directly affect cell behavior, making it difficult to distinguish purely mechanical signaling events. This study reports on the development of a hydrogel that incorporates photoswitchable cross-linkers, which can reversibly alter their stiffness upon irradiation with the appropriate wavelength of light. Furthermore, this study reports the response of bone-marrow-derived mesenchymal stem cells (MSCs) on these hydrogels that were stiffened systematically by irradiation with blue light. The substrates were shown to be noncytotoxic, and crucially MSCs were not affected by blue-light exposure. Time-resolved analysis of cell morphology showed characteristic cell spreading and increased aspect ratios in response to greater substrate stiffness. This hydrogel provides a platform to study mechanosignaling in cells responding to dynamic changes in stiffness, offering a new way to study mechanotransduction signaling pathways and biological processes, with implicit changes to tissue mechanics, such as development, ageing, and fibrosis.

Item Type: Article
Uncontrolled Keywords: photoswitchable cross-linkers, hydrogel, azobenzene, mesenchymal stem cells, morphometrics, mechanotransduction
Depositing User: Symplectic Admin
Date Deposited: 28 Feb 2018 13:59
Last Modified: 19 Jan 2023 06:38
DOI: 10.1021/acsami.7b18302
Related URLs:
URI: https://livrepository.liverpool.ac.uk/id/eprint/3018480