HRS-WASH axis governs Actin mediated endosomal recycling and cell invasion

MacDonald, Ewan, Brown, louise, Selvais, A ORCID: 0000-0002-1874-7853, Liu, H, Waring, thomas ORCID: 0000-0002-3280-8886, Newman, Daniel ORCID: 0000-0003-0534-8914, Bithell, Jessica, Grimes, douglas ORCID: 0000-0002-1034-6625, Urbe, sylvie ORCID: 0000-0003-4735-9814, Clague, MJ ORCID: 0000-0003-3355-9479
et al (show 1 more authors) (2018) HRS-WASH axis governs Actin mediated endosomal recycling and cell invasion. The Journal of Cell Biology, 217 (7). pp. 2549-2564.

[thumbnail of 47565_2_merged_1524765216.pdf] Text
47565_2_merged_1524765216.pdf - Author Accepted Manuscript

Download (206MB)


Transmembrane proteins in the sorting endosome are either recycled to their point of origin or destined for lysosomal degradation. Lysosomal sorting is mediated by interaction of ubiquitylated transmembrane proteins with the endosomal sorting complex required for transport (ESCRT) machinery. In this study, we uncover an alternative role for the ESCRT-0 component hepatocyte growth factor–regulated tyrosine kinase substrate (HRS) in promoting the constitutive recycling of transmembrane proteins. We find that endosomal localization of the actin nucleating factor Wiscott-Aldrich syndrome protein and SCAR homologue (WASH) requires HRS, which occupies adjacent endosomal subdomains. Depletion of HRS results in defective constitutive recycling of epidermal growth factor receptor and the matrix metalloproteinase MT1–MMP, leading to their accumulation in internal compartments. We show that direct interactions with endosomal actin are required for efficient recycling and use a model system of chimeric transferrin receptor trafficking to show that an actin-binding motif can counteract an ubiquitin signal for lysosomal sorting. Directed receptor recycling is used by cancer cells to achieve invasive migration. Accordingly, abrogating HRS- and actin-dependent MT1-MMP recycling results in defective matrix degradation and invasion of triple-negative breast cancer cells.

Item Type: Article
Uncontrolled Keywords: biochemistry, cancer, trafficking
Depositing User: Symplectic Admin
Date Deposited: 16 May 2018 15:15
Last Modified: 19 Jan 2023 06:33
DOI: 10.1083/jcb.201710051
Related URLs: