Noninvasive imaging of nanoparticle-labeled transplant populations within polymer matrices for neural cell therapy

Tickle, Jacqueline A, Poptani, Harish ORCID: 0000-0002-0593-3235, Taylor, Arthur ORCID: 0000-0003-2028-6694 and Chari, Divya M (2018) Noninvasive imaging of nanoparticle-labeled transplant populations within polymer matrices for neural cell therapy. NANOMEDICINE, 13 (11). pp. 1333-1348.

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Official URL: http://dx.doi.org/10.2217/nnm-2017-0347

Abstract

<h4>Aim</h4>To develop a 3D neural cell construct for encapsulated delivery of transplant cells; develop hydrogels seeded with magnetic nanoparticle (MNP)-labeled cells suitable for cell tracking by MRI.<h4>Materials & methods</h4>Astrocytes were exogenously labeled with MRI-compatible iron-oxide MNPs prior to intra-construct incorporation within a 3D collagen hydrogel.<h4>Results</h4>A connective, complex cellular network was clearly observable within the 3D constructs, with high cellular viability. MNP accumulation in astrocytes provided a hypointense MRI signal at 24 h & 14 days.<h4>Conclusion</h4>Our findings support the concept of developing a 3D construct possessing the dual advantages of (i) support of long-term cell survival of neural populations with (ii) the potential for noninvasive MRI-tracking of intra-construct cells for neuroregenerative applications.

Item Type:	Article
Uncontrolled Keywords:	3D collagen hydrogel, astrocytes, MRI, transmission electron microscopy
Depositing User:	Symplectic Admin
Date Deposited:	17 Jul 2018 09:57
Last Modified:	15 Mar 2024 02:37
DOI:	10.2217/nnm-2017-0347
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3023670