Wood, O, Woo, J, Seumois, G, Savelyeva, N, McCann, KJ, Singh, D, Jones, T, Peel, L, Breen, MS, Ward, M et al (show 8 more authors)
(2016)
Gene expression analysis of TIL rich HPV-driven head and neck tumors reveals a distinct B-cell signature when compared to HPV independent tumors.
Oncotarget, 7 (35).
pp. 56781-56797.
Abstract
Human papilloma virus (HPV)-associated head and neck squamous cell carcinoma (HNSCC) has a better prognosis than it’s HPV negative (HPV(−)) counterpart. This may be due to the higher numbers of tumor-infiltrating lymphocytes (TILs) in HPV positive (HPV(+)) tumors. RNA-Sequencing (RNA-Seq) was used to evaluate whether the differences in clinical behaviour simply reflect a numerical difference in TILs or whether there is a fundamental behavioural difference between TILs in these two settings. Thirty-nine HNSCC tumors were scored for TIL density by immunohistochemistry. After the removal of 16 TILlow tumors, RNA-Seq analysis was performed on 23 TILhigh/med tumors (HPV(+) n=10 and HPV(−) n=13). Using EdgeR, differentially expressed genes (DEG) were identified. Immune subset analysis was performed using Functional Analysis of Individual RNA-Seq/ Microarray Expression (FAIME) and immune gene RNA transcript count analysis. In total, 1,634 DEGs were identified, with a dominant immune signature observed in HPV(+) tumors. After normalizing the expression profiles to account for differences in B- and T-cell number, 437 significantly DEGs remained. A B-cell associated signature distinguished HPV(+) from HPV(−) tumors, and included the DEGs CD200, GGA2, ADAM28, STAG3, SPIB, VCAM1, BCL2 and ICOSLG; the immune signal relative to T-cells was qualitatively similar between TILs of both tumor cohorts. Our findings were validated and confirmed in two independent cohorts using TCGA data and tumor-infiltrating B-cells from additional HPV(+) HNSCC patients. A B-cell associated signal segregated tumors relative to HPV status. Our data suggests that the role of B-cells in the adaptive immune response to HPV(+) HNSCC requires re-assessment.
Item Type: | Article |
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Uncontrolled Keywords: | head and neck squamous cell carcinoma, human papilloma virus, tumor-infiltrating lymphocyte, RNA-sequencing, transcriptome |
Depositing User: | Symplectic Admin |
Date Deposited: | 24 Jul 2018 06:26 |
Last Modified: | 19 Jan 2023 01:30 |
DOI: | 10.18632/oncotarget.10788 |
Open Access URL: | https://doi.org/10.18632/oncotarget.10788 |
Related URLs: | |
URI: | https://livrepository.liverpool.ac.uk/id/eprint/3024086 |