Zika Virus Infection Preferentially Counterbalances Human Peripheral Monocyte and/or NK Cell Activity


Lum, Fok-Moon, Lee, David, Chua, Tze-Kwang, Tan, Jeslin JL, Lee, Cheryl YP, Liu, Xuan, Fang, Yongxiang, Lee, Bernett, Yee, Wearn-Xin, Rickett, Natasha Y et al (show 6 more authors) (2018) Zika Virus Infection Preferentially Counterbalances Human Peripheral Monocyte and/or NK Cell Activity. MSPHERE, 3 (2).

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Abstract

Zika virus (ZIKV) has reemerged in the population and caused unprecedented global outbreaks. Here, the transcriptomic consequences of ZIKV infection were studied systematically first in human peripheral blood CD14+ monocytes and monocyte-derived macrophages with high-density RNA sequencing. Analyses of the ZIKV genome revealed that the virus underwent genetic diversification, and differential mRNA abundance was found in host cells during infection. Notably, there was a significant change in the cellular response, with cross talk between monocytes and natural killer (NK) cells as one of the highly identified pathways. Immunophenotyping of peripheral blood from ZIKV-infected patients further confirmed the activation of NK cells during acute infection. ZIKV infection in peripheral blood cells isolated from healthy donors led to the induction of gamma interferon (IFN-γ) and CD107a—two key markers of NK cell function. Depletion of CD14+ monocytes from peripheral blood resulted in a reduction of these markers and reduced priming of NK cells during infection. This was complemented by the immunoproteomic changes observed. Mechanistically, ZIKV infection preferentially counterbalances monocyte and/or NK cell activity, with implications for targeted cytokine immunotherapies.

Item Type: Article
Uncontrolled Keywords: NK cells, RNA-seq, Zika virus, immune response, monocytes, transcriptomes
Depositing User: Symplectic Admin
Date Deposited: 25 Jan 2019 09:37
Last Modified: 19 Jan 2023 01:06
DOI: 10.1128/mSphereDirect.00120-18
Related URLs:
URI: https://livrepository.liverpool.ac.uk/id/eprint/3031740
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