Govaert, Marlies, Smet, Cindy, Baka, Maria, Ecimovic, Branimir, Walsh, James L 
ORCID: 0000-0002-6318-0892 and Van Impe, Jan
(2018)
Resistance of L. monocytogenes and S. Typhimurium towards Cold Atmospheric Plasma as Function of Biofilm Age.
APPLIED SCIENCES-BASEL, 8 (12).
p. 2702.
Abstract
<jats:p>The biofilm mode of growth protects bacterial cells against currently applied disinfection methods for abiotic (food) contact surfaces. Therefore, innovative methods, such as Cold Atmospheric Plasma (CAP), should be investigated for biofilm inactivation. However, more knowledge is required concerning the influence of the biofilm age on the inactivation efficacy in order to comment on a possible application of CAP in the (food) processing industry. L. monocytogenes and S. Typhimurium biofilms with five different ages (i.e., 1, 2, 3, 7, and 10 days) were developed. For the untreated biofilms, the total biofilm mass and the cell density were determined. To investigate the biofilm resistance towards CAP treatment, biofilms with different ages were treated for 10 min and the remaining cell density was determined. Finally, for the one-day old reference biofilms and the most resistant biofilm age, complete inactivation curves were developed to examine the influence of the biofilm age on the inactivation kinetics. For L. monocytogenes, an increased biofilm age resulted in (i) an increased biomass, (ii) a decreased cell density prior to CAP treatment, and (iii) an increased resistance towards CAP treatment. For S. Typhimurium, similar results were obtained, except for the biomass, which was here independent of the biofilm age.</jats:p>
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Listeria monocytogenes, Salmonella Typhimurium, biofilm age, Cold Atmospheric Plasma, plate counts, crystal violet assay |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 13 Feb 2019 11:47 |
| Last Modified: | 19 Jan 2023 01:04 |
| DOI: | 10.3390/app8122702 |
| Open Access URL: | https://doi.org/10.3390/app8122702 |
| Related URLs: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3032697 |
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