<i>Trypanosoma brucei</i>colonises the tsetse gut via an immature peritrophic matrix in the proventriculus



Rose, Clair ORCID: 0000-0001-7782-5359, Dyer, Naomi, Casas-Sanchez, Aitor ORCID: 0000-0001-5237-1223, Beckett, Alison, Solórzano, Carla, Middlehurst, Ben, Marcello, Marco ORCID: 0000-0002-2392-8640, Lehane, Michael, Prior, Ian ORCID: 0000-0002-4055-5161 and Acosta-Serrano, Álvaro
(2019) <i>Trypanosoma brucei</i>colonises the tsetse gut via an immature peritrophic matrix in the proventriculus. Preprint. 513689-.

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Abstract

The peritrophic matrix (PM) of haematophagus insects is a chitinous structure that surrounds the bloodmeal, forming a protective barrier against oral pathogens and abrasive particles. To establish an infection in the tsetse midgut, Trypanosoma brucei must colonise the ectoperitrophic space (ES), located between the PM and gut epithelium. Although unproven, it is generally accepted that trypanosomes reach the ES by directly penetrating the PM in the anterior midgut. Here we revisited this event by employing novel fluorescence and electron microscopy methodologies and found that instead, trypanosomes reach the ES via the newly secreted PM in the tsetse proventriculus. Within this model, parasites colonising the proventriculus can either migrate to the ES or become trapped within PM layers forming cysts that move along the entire gut as the PM gets remodelled. Early proventricular colonisation appears to be promoted by unidentified factors in trypanosome-infected blood, resulting in higher salivary gland infections and potentially increasing parasite transmission.

Item Type: Article
Uncontrolled Keywords: Vector-Borne Diseases, Infectious Diseases, Rare Diseases, 2 Aetiology, 2.2 Factors relating to the physical environment, Infection, 3 Good Health and Well Being
Depositing User: Symplectic Admin
Date Deposited: 13 Mar 2019 15:43
Last Modified: 14 Mar 2024 18:38
DOI: 10.1101/513689
Related URLs:
URI: https://livrepository.liverpool.ac.uk/id/eprint/3033563