Comparative 1D Blue-Native electrophoresis analysis of <i>Plasmodium falciparum</i> and human proteins associated with cytoadherence



Wu, Yang, Wagstaff, Simon C, Al-Harthi, Saeed A and Craig, Alister G ORCID: 0000-0003-0914-6164
(2018) Comparative 1D Blue-Native electrophoresis analysis of <i>Plasmodium falciparum</i> and human proteins associated with cytoadherence. MALARIA JOURNAL, 17 (1). 293-.

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Abstract

<h4>Background</h4>To understand more about changes to the molecular components that occur when host endothelium interacts with Plasmodium falciparum-infected erythrocytes, a combined technique of protein separation (1D Blue-Native electrophoresis) and mass spectrometry of infected erythrocytes with endothelial cells (EC) in a co-culture system has been used.<h4>Methods</h4>Native proteins were extracted from co-cultures and identified by mass spectrometry. Proteomic data from different parasite strains, either adhesion proficient (to endothelial cells) or non-adherent, were analysed in parallel to reveal protein associations linked to cytoadherence. Informatic approaches were developed to facilitate this comparison.<h4>Results</h4>Blue-Native gel separation and LC/MS/MS identification revealed major differences in samples produced from endothelial cell co-culture with adherent and non-adherent parasite strains. This approach enabled us to identify protein associations seen only with the adhesion proficient parasite strain.<h4>Conclusions</h4>The combination of proteomic and analytical approaches has identified differences between adherent and non-adherent parasite lines in co-culture with EC, providing potential candidates for complexes or associations formed during cytoadherence involved in cell structure, signalling and apoptosis.

Item Type: Article
Uncontrolled Keywords: Plasmodium falciparum, Cytoadherence, Blue native electrophoresis, Protein complexes
Depositing User: Symplectic Admin
Date Deposited: 13 May 2019 10:51
Last Modified: 07 Oct 2023 14:24
DOI: 10.1186/s12936-018-2445-8
Open Access URL: https://doi.org/10.1186/s12936-018-2445-8
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URI: https://livrepository.liverpool.ac.uk/id/eprint/3041055