Molina-Moya, Barbara, Abdurrahman, Saddiq T, Madukaji, Laura I, Gomgnimbou, Michel Kireopori, Spinasse, Lizania, Gomes-Fernandes, Meissiner, Gomes, Harrison Magdinier, Kacimi, Sarah, Dacombe, Russell, Bimba, John S et al (show 4 more authors)
(2018)
Genetic characterization of Mycobacterium tuberculosis complex isolates circulating in Abuja, Nigeria.
INFECTION AND DRUG RESISTANCE, 11.
pp. 1617-1625.
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Abstract
<h4>Objective</h4>Nigeria ranks fourth among the high tuberculosis (TB) burden countries. This study describes the prevalence of drug resistance and the genetic diversity of <i>Mycobacterium tuberculosis</i> in Abuja's Federal Capital Territory.<h4>Materials and methods</h4>Two hundred and seventy-eight consecutive sputum samples were collected from adults with presumptive TB during 2013-2014. DNA was extracted from Löwenstein-Jensen cultures and analyzed for the identification of nontuberculous mycobacteria species, detection of drug resistance with line probe assays, and high-throughput spacer oligonucleotide typing (spoligotyping) using microbead-based hybridization.<h4>Results</h4>Two hundred and two cultures were positive for <i>M. tuberculosis</i> complex, 24 negative, 38 contaminated, and 15 positive for nontuberculous mycobacteria. Five (2.5%) <i>M. tuberculosis</i> complex isolates were resistant to rifampicin (RIF) and isoniazid (multidrug resistant), nine (4.5%) to RIF alone, and 15 (7.4%) to isoniazid alone; two RIF-resistant isolates were also resistant to fluoroquinolones and ethambutol, and one multidrug resistant isolate was also resistant to ethambutol. Among the 180 isolates with spoligotyping results, 164 (91.1%) were classified as lineage 4 (Euro-American), 13 (7.2%) as lineage 5 (West African 1), two (1.1%) as lineage 2 (East Asia), and one (0.6%) as lineage 6 (West African 2). One hundred and fifty-six (86.7%) isolates were grouped in 17 clusters (2-108 isolates/cluster), of which 108 (60.0%) were grouped as L4.6.2/Cameroon (spoligotype international type 61).<h4>Conclusion</h4>The description of drug resistance prevalence and genetic diversity of <i>M. tuberculosis</i> in this study may be useful for improving TB control in Nigeria.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | tuberculosis, isoniazid, rifampicin, line probe assay, microbeads, spoligotyping |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 14 May 2019 10:45 |
| Last Modified: | 19 Jan 2023 00:45 |
| DOI: | 10.2147/IDR.S166986 |
| Open Access URL: | https://doi.org/10.2147/IDR.S166986 |
| Related URLs: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3041250 |
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