Next generation histology methods for three-dimensional imaging of fresh and archival human brain tissues



Lai, Hei Ming, Liu, Alan King Lun, Ng, Harry Ho Man, Goldfinger, Marc H, Chau, Tsz Wing, DeFelice, John, Tilley, Bension S, Wong, Wai Man, Wu, Wutian and Gentleman, Steve M
(2018) Next generation histology methods for three-dimensional imaging of fresh and archival human brain tissues. NATURE COMMUNICATIONS, 9 (1). 1066-.

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Abstract

Modern clearing techniques for the three-dimensional (3D) visualisation of neural tissue microstructure have been very effective when used on rodent brain but very few studies have utilised them on human brain material, mainly due to the inherent difficulties in processing post-mortem tissue. Here we develop a tissue clearing solution, OPTIClear, optimised for fresh and archival human brain tissue, including formalin-fixed paraffin-embedded material. In light of practical challenges with immunostaining in tissue clearing, we adapt the use of cresyl violet for visualisation of neurons in cleared tissue, with the potential for 3D quantification in regions of interest. Furthermore, we use lipophilic tracers for tracing of neuronal processes in post-mortem tissue, enabling the study of the morphology of human dendritic spines in 3D. The development of these different strategies for human tissue clearing has wide applicability and, we hope, will provide a baseline for further technique development.

Item Type: Article
Uncontrolled Keywords: Brain, Humans, Catecholamines, Imaging, Three-Dimensional, Fluorescent Antibody Technique, Immunohistochemistry, Paraffin Embedding
Depositing User: Symplectic Admin
Date Deposited: 31 May 2019 09:24
Last Modified: 19 Jan 2023 00:42
DOI: 10.1038/s41467-018-03359-w
Open Access URL: https://doi.org/10.1038/s41467-018-03359-w
Related URLs:
URI: https://livrepository.liverpool.ac.uk/id/eprint/3043811