Reservoirs of resistance: polymyxin resistance in veterinary-associated companion animal isolates of <i>Pseudomonas aeruginosa</i>

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Scott, Andrea, Pottenger, Sian ORCID: 0000-0002-5700-9693, Timofte, Donna ORCID: 0000-0002-7261-738X, Moore, Matthew, Wright, Laura ORCID: 0000-0002-0358-5783, Kukavica-Ibrulj, Irena, Jeukens, Julie, Levesque, Roger C, Freschi, Luca, Pinchbeck, Gina L ORCID: 0000-0002-5671-8623 et al (show 4 more authors) , Schmidt, Vanessa M ORCID: 0000-0001-5460-6217, McEwan, Neil, Radford, Alan D ORCID: 0000-0002-4590-1334 and Fothergill, Joanne L ORCID: 0000-0002-7012-1508 (2019) Reservoirs of resistance: polymyxin resistance in veterinary-associated companion animal isolates of <i>Pseudomonas aeruginosa</i>. VETERINARY RECORD, 185 (7). 206-.

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Abstract

<h4>Background</h4><i>Pseudomonas aeruginosa</i> is an opportunistic pathogen and a major cause of infections. Widespread resistance in human infections are increasing the use of last resort antimicrobials such as polymyxins. However, these have been used for decades in veterinary medicine. Companion animals are an understudied source of antimicrobial resistant <i>P. aeruginosa</i> isolates. This study evaluated the susceptibility of <i>P. aeruginosa</i> veterinary isolates to polymyxins to determine whether the veterinary niche represents a potential reservoir of resistance genes for pathogenic bacteria in both animals and humans.<h4>Methods and results</h4>Clinical <i>P. aeruginosa</i> isolates (n=24) from UK companion animals were compared for antimicrobial susceptibility to a panel of human-associated isolates (n=37). Minimum inhibitory concentration (MIC) values for polymyxin B and colistin in the companion animals was significantly higher than in human isolates (P=0.033 and P=0.013, respectively). Genotyping revealed that the veterinary isolates were spread throughout the <i>P. aeruginosa</i> population, with shared array types from human infections such as keratitis and respiratory infections, suggesting the potential for zoonotic transmission. Whole genome sequencing revealed mutations in genes associated with polymyxin resistance and other antimicrobial resistance-related genes.<h4>Conclusion</h4>The high levels of resistance to polymyxin shown here, along with genetic similarities between some human and animal isolates, together suggest a need for sustained surveillance of this veterinary niche as a potential reservoir for resistant, clinically relevant bacteria in both animals and humans.

Item Type:	Article
Uncontrolled Keywords:	Animals, Humans, Pseudomonas aeruginosa, Polymyxins, Veterinary Medicine, Drug Resistance, Bacterial, Pets, United Kingdom
Depositing User:	Symplectic Admin
Date Deposited:	19 Jun 2019 12:24
Last Modified:	12 Oct 2023 09:00
DOI:	10.1136/vr.105075
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3046568

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• Reservoirs of resistance: polymyxin resistance in veterinary-associated companion animal isolates of Pseudomonas aeruginosa. (deposited 05 Jun 2019 15:48)
  • Reservoirs of resistance: polymyxin resistance in veterinary-associated companion animal isolates of <i>Pseudomonas aeruginosa</i>. (deposited 19 Jun 2019 12:24) [Currently Displayed]