Scavenius, Carsten, Poulsen, Emil Christian, Thøgersen, Ida B, Roebuck, Margaret ORCID: 0000-0002-1193-5149, Frostick, Simon, Bou-Gharios, George, Yamamoto, Kazuhiro ORCID: 0000-0002-8481-775X, Deleuran, Bent and Enghild, Jan J
(2019)
Matrix-degrading protease ADAMTS-5 cleaves inter-α-inhibitor and releases active heavy chain 2 in synovial fluids from arthritic patients.
Journal of Biological Chemistry, 294 (42).
pp. 15495-15504.
Text
J. Biol. Chem.-2019-Scavenius-jbc.RA119.008844.pdf - Published version Download (373kB) | Preview |
Abstract
Destruction of the cartilage matrix in joints is an important feature of arthritis. Proteolytic degradation of cartilage glycoproteins can contribute to the loss of matrix integrity. Human inter-α-inhibitor (IαI), which stabilizes the extracellular matrix, is composed of the light-chain serine proteinase inhibitor bikunin and two homologous heavy chains (HC1 and HC2) covalently linked through chondroitin 4-sulfate. Inflammation promotes the transfer of HCs from chondroitin 4-sulfate to hyaluronan by tumor necrosis factor-stimulated gene-6 protein (TSG-6). This reaction generates a covalent complex between the heavy chains and hyaluronan that can promote leukocyte invasion. This study demonstrates that both IαI and the HC-hyaluronan complex are substrates for the extracellular matrix proteases ADAMTS-5 and matrix metalloprotease (MMP) -3, -7, and -13. The major cleavage sites for all four proteases are found in the C terminus of HC2. ADAMTS-5 and MMP-7 displayed the highest activity toward HC2. ADAMTS-5 degradation products were identified in mass spectrometric analysis of 29 of 33 arthropathic patients, indicating that ADAMTS-5 cleavage occurs in synovial fluid in arthritis. After cleavage, free HC2, together with TSG-6, is able to catalyze the transfer of heavy chains to hyaluronan. The release of extracellular matrix bound HC2 is likely to increase the mobility of the HC2/TSG-6 catalytic unit and consequently increase the rate of the HC transfer reaction. Ultimately, ADAMTS-5 cleavage of HC2 could alter the physiological and mechanical properties of the extracellular matrix and contribute to the progression of arthritis.
Item Type: | Article |
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Uncontrolled Keywords: | arthritis, hyaluronan, ADAMTS, extracellular matrix protein, metalloprotease, bikunin, inter-α-inhibitor |
Depositing User: | Symplectic Admin |
Date Deposited: | 12 Sep 2019 14:06 |
Last Modified: | 19 Jan 2023 00:26 |
DOI: | 10.1074/jbc.ra119.008844 |
Related URLs: | |
URI: | https://livrepository.liverpool.ac.uk/id/eprint/3054352 |