Small Non-Coding RNAome of Ageing Chondrocytes

Balaskas, Panagiotis, Green, Jonathan A, Haqqi, Tariq M, Dyer, Philip, Kharaz, Yalda A, Fang, Yongxiang ORCID: 0000-0002-4514-5292, Liu, Xuan, Welting, Tim Jm and Peffers, Mandy J ORCID: 0000-0001-6979-0440 (2020) Small Non-Coding RNAome of Ageing Chondrocytes. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 21 (16). E5675-.

Access the full-text of this item by clicking on the Open Access link.

Official URL: http://dx.doi.org/10.3390/ijms21165675

Abstract

Ageing is a leading risk factor predisposing cartilage to osteoarthritis. However, little research has been conducted on the effect of ageing on the expression of small non-coding RNAs (sncRNAs). RNA from young and old chondrocytes from macroscopically normal equine metacarpophalangeal joints was extracted and subjected to small RNA sequencing (RNA-seq). Differential expression analysis was performed in R using package DESeq2. For transfer RNA (tRNA) fragment analysis, tRNA reads were aligned to horse tRNA sequences using Bowtie2 version 2.2.5. Selected microRNA (miRNAs or miRs) and small nucleolar RNA (snoRNA) findings were validated using real-time quantitative Polymerase Chain Reaction (qRT-PCR) in an extended cohort of equine chondrocytes. tRNA fragments were further investigated in low- and high-grade OA human cartilage tissue. In total, 83 sncRNAs were differentially expressed between young and old equine chondrocytes, including miRNAs, snoRNAs, small nuclear RNAs (snRNAs), and tRNAs. qRT-PCR analysis confirmed findings. tRNA fragment analysis revealed that tRNA halves (tiRNAs), tiRNA-5035-GluCTC and tiRNA-5031-GluCTC-1 were reduced in both high grade OA human cartilage and old equine chondrocytes. For the first time, we have measured the effect of ageing on the expression of sncRNAs in equine chondrocytes. Changes were detected in a number of different sncRNA species. This study supports a role for sncRNAs in ageing cartilage and their potential involvement in age-related cartilage diseases.

Item Type:	Article
Uncontrolled Keywords:	chondrocyte, ageing, equine, small non-coding RNA
Depositing User:	Symplectic Admin
Date Deposited:	12 Aug 2020 14:34
Last Modified:	20 Jan 2024 09:20
DOI:	10.3390/ijms21165675
Open Access URL:	https://doi.org/10.3390/ijms21165675
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3097317