Vulpeculin: a novel and abundant lipocalin in the urine of the common brushtail possum, Trichosurus vulpecula.

Loxley, Grace M, Hooks, David O, Antonopoulos, Aristotelis, Dell, Anne, Haslam, Stuart M, Linklater, Wayne L, Hurst, Jane L ORCID: 0000-0002-3728-9624 and Beynon, Robert J ORCID: 0000-0003-0857-495X (2020) Vulpeculin: a novel and abundant lipocalin in the urine of the common brushtail possum, Trichosurus vulpecula. Open Biology, 10 (10). p. 200218.

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Abstract

Lipocalins are a family of secreted proteins. They are capable of binding small lipophilic compounds and have been extensively studied for their role in chemosignalling in rodent urine. Urine of the common brushtail possum (Trichosurus vulpecula) contains a prominent glycoprotein of 20 kDa, expressed in both sexes. We have isolated this protein and determined its primary sequence by mass spectrometry, including the use of metabolic labelling to resolve the leucine/isoleucine isobaric ambiguity. The protein sequence was identified as a lipocalin, and phylogenetic analysis grouped the protein with other marsupial lipocalin sequences in a phylogenetic clade distinct from established cross-species lipocalin sub-families. The pattern of expression in possum urine and the similarity in sequence and structure to other lipocalins suggests this protein may have a role in brushtail possum chemosignalling.

Item Type:	Article
Uncontrolled Keywords:	brushtail possum, Trichosurus vulpecula, major urinary protein, metabolic labelling, mass spectrometry
Depositing User:	Symplectic Admin
Date Deposited:	19 Oct 2020 09:27
Last Modified:	18 Jan 2023 23:28
DOI:	10.1098/rsob.200218
Open Access URL:	https://royalsocietypublishing.org/doi/10.1098/rso...
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3104449