Minimising damage in high resolution scanning transmission electron microscope images of nanoscale structures and processes.



Nicholls, Daniel ORCID: 0000-0003-1677-701X, Lee, Juhan ORCID: 0000-0001-5036-3449, Amari, Houari, Stevens, Andrew J, Mehdi, B Layla ORCID: 0000-0002-8281-9524 and Browning, Nigel D ORCID: 0000-0003-0491-251X
(2020) Minimising damage in high resolution scanning transmission electron microscope images of nanoscale structures and processes. Nanoscale, 12 (41). pp. 21248-21254.

[img] Text
NichollsetalNanoscale2020Final_Revision1.pdf - Author Accepted Manuscript

Download (1MB) | Preview

Abstract

Beam damage caused during acquisition of the highest resolution images is the current limitation in the vast majority of experiments performed in a scanning transmission electron microscope (STEM). While the principles behind the processes of knock-on and radiolysis damage are well-known (as are other contributing effects, such as heat and electric fields), understanding how and especially when beam damage is distributed across the entire sample volume during an experiment has not been examined in detail. Here we use standard models for damage and diffusion to elucidate how beam damage spreads across the sample as a function of the microscope conditions to determine an "optimum" sampling approach that maximises the high-resolution information in any image acquisition. We find that the standard STEM approach of scanning an image sequentially accelerates damage because of increased overlap of diffusion processes. These regions of accelerated damage can be significantly decelerated by increasing the distance between the acquired pixels in the scan, forming a "spotscan" mode of acquisition. The optimum distance between these pixels can be broadly defined by the fundamental properties of each material, allowing experiments to be designed for specific beam sensitive materials. As an added bonus, if we use inpainting to reconstruct the sparse distribution of pixels in the image we can significantly increase the speed of the STEM process, allowing dynamic phenomena, and the onset of damage, to be studied directly.

Item Type: Article
Uncontrolled Keywords: Stem Cell Research
Depositing User: Symplectic Admin
Date Deposited: 22 Oct 2020 09:43
Last Modified: 15 Mar 2024 13:39
DOI: 10.1039/d0nr04589f
Related URLs:
URI: https://livrepository.liverpool.ac.uk/id/eprint/3104865