Suitable disk antimicrobial susceptibility breakpoints defining Salmonella enterica serovar Typhi isolates with reduced susceptibility to fluoroquinolones.



Parry, Christopher M ORCID: 0000-0001-7563-7282, Thuy, Chau Tran, Dongol, Sabina, Karkey, Abhilasha, Vinh, Ha, Chinh, Nguyen Tran, Duy, Pham Thanh, Thieu Nga, Tran Vu, Campbell, James I, Van Minh Hoang, Nguyen
et al (show 15 more authors) (2010) Suitable disk antimicrobial susceptibility breakpoints defining Salmonella enterica serovar Typhi isolates with reduced susceptibility to fluoroquinolones. Antimicrobial agents and chemotherapy, 54 (12). pp. 5201-5208.

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Abstract

Infections with Salmonella enterica serovar Typhi isolates that have reduced susceptibility to ofloxacin (MIC ≥ 0.25 μg/ml) or ciprofloxacin (MIC ≥ 0.125 μg/ml) have been associated with a delayed response or clinical failure following treatment with these antimicrobials. These isolates are not detected as resistant using current disk susceptibility breakpoints. We examined 816 isolates of S. Typhi from seven Asian countries. Screening for nalidixic acid resistance (MIC ≥ 16 μg/ml) identified isolates with an ofloxacin MIC of ≥0.25 μg/ml with a sensitivity of 97.3% (253/260) and specificity of 99.3% (552/556). For isolates with a ciprofloxacin MIC of ≥0.125 μg/ml, the sensitivity was 92.9% (248/267) and specificity was 98.4% (540/549). A zone of inhibition of ≤28 mm around a 5-μg ofloxacin disc detected strains with an ofloxacin MIC of ≥0.25 μg/ml with a sensitivity of 94.6% (246/260) and specificity of 94.2% (524/556). A zone of inhibition of ≤30 mm detected isolates with a ciprofloxacin MIC of ≥0.125 μg/ml with a sensitivity of 94.0% (251/267) and specificity of 94.2% (517/549). An ofloxacin MIC of ≥0.25 μg/ml and a ciprofloxacin MIC of ≥0.125 μg/ml detected 74.5% (341/460) of isolates with an identified quinolone resistance-inducing mutation and 81.5% (331/406) of the most common mutant (carrying a serine-to-phenylalanine mutation at codon 83 in the gyrA gene). Screening for nalidixic acid resistance or ciprofloxacin and ofloxacin disk inhibition zone are suitable for detecting S. Typhi isolates with reduced fluoroquinolone susceptibility.

Item Type: Article
Uncontrolled Keywords: Humans, Salmonella typhi, Nalidixic Acid, Fluoroquinolones, Ciprofloxacin, Ofloxacin, DNA Gyrase, DNA Topoisomerase IV, Bacterial Proteins, Anti-Bacterial Agents, Microbial Sensitivity Tests, Drug Resistance, Bacterial, Mutation
Depositing User: Symplectic Admin
Date Deposited: 18 Nov 2020 09:32
Last Modified: 18 Mar 2024 01:03
DOI: 10.1128/aac.00963-10
Related URLs:
URI: https://livrepository.liverpool.ac.uk/id/eprint/3107297